Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus

作     者：Tenggen Hu Yuanshan Yu Jijun Wu Yujuan Xu Gengsheng Xiao Kejing An Erna Li Sentai Liao Yuxiao Zou Tenggen Hu;Yuanshan Yu;Jijun Wu;Yujuan Xu;Gengsheng Xiao;Kejing An;Erna Li;Sentai Liao;Yuxiao Zou

作者机构：Sericultural&Agri-Food Research InstituteGuangdong Academy of Agricultural Sciences/Key Laboratory of Functional FoodsMinistry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China Huagongliya(Foshan)Technology Industry Co.Ltd.Foshan 528313China Heyuan BranchGuangdong Laboratory for Lingnan Modern AgricultureHeyuan 517001China Zhongkai University of Agriculture and EngineeringGuangzhou 510225China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2024年第13卷第4期

页      面：2161-2173页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 083201[工学-食品科学] 

基　　金：the Key Research and Development Program of Yunnan Province(No.202202AE090023) Key Research and Development Program of Guangdong Province(No.2022B0202040002 2022B0202050001) the Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculture the Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture Project(No.DT20220026) Talent Introduction Program of Guangdong Academy of Agricultural Sciences(No.R2022YJ-YB3004) the Basic and Applied Basic Research Project of Guangdong Province(No.2022A1515110227 2023A1515012386) the Science and Technology Planning Project of Guangzhou(No.2023A04J0828) the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams(No.202109TD) the Special Fund Project for Teachers’Scientific and Technological Achievements Transformation in Shunde Innovation Park,National University Science Park,South China University of Technology(No.KJYS2021KZ05)for their financial support 

主　　题：Mulberry leaf oligosaccharide Characterization Gut microbiota Lactobacillus murinus Hypoglycemic activity 

摘      要：Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf *** results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut ***,MLO 2-1 was selected for the further *** structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-*** in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut ***,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and *** specific metabolic pathways of MLO 2-1 by gut microbiota were further *** microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM *** experiments indicated that MLO 2-1 and *** exhibited hypoglycemic *** results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of ***.