Study on the Action Mechanism of Euphorbia peplus in the Treatment of Alzheimer s Disease Based on UPLC-Q-TOF-MS/MS Combined with Network Pharmacology and Molecular Docking
作者机构：People’s Hospital of Anshun City Guizhou ProvinceAnshun 561000China
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2023年第12卷第4期
页 面：122-131页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：Euphorbia peplus Alzheimer s disease Network pharmacology Molecular docking
摘 要：[Objectives]Based on UPLC-Q-TOF-MS/MS,network pharmacology and molecular docking techniques,the mechanism of Euphorbia peplus in the treatment of Alzheimer s disease(AD)was studied.[Methods]The UPLC-Q-TOF-MS/MS technique was used to rapidly analyze the chemical components of *** components and potential targets of *** were retrieved from TCMSP and Swiss Target Prediction database,and AD targets were screened using GeneCards *** targets of *** in the treatment of AD were *** PPI network was constructed using String platform,and the network topology of Cytoscape software was used to compute and screen key targets,and the GO and KEGG pathway enrichment analysis was carried out in Metascape database to construct thecomponent-target-pathway-disease*** docking was used to predict the binding properties of active ingredients and targets.[Results]The results of UPLC-Q-TOF-MS/MS showed that 83 compounds were identified from ***,including 19 terpenoids,10 phenolic acids and phenols,16 flavonoids,2 phenylpropanoids,4 coumarins,1 alkaloid,1 anthraquinone and 30 other *** results of network pharmacological analysis showed that 82 active ingredients were screened,and 279 common targets were identified for the treatment of AD,among which the key targets were ALB(albumin),GAPDH(glyceraldehyde triphosphate dehydrogenase),TNF(tumor necrosis factor),AKT1(serine/threonine protein kinase 1),and IL6(interleukin-6).KEGG enrichment analysis showed that key signaling pathways include cancer pathways,lipid and atherosclerosis,Alzheimer s disease,insulin resistance,serotonergic synapses,calcium signaling pathway,cAMP signaling pathway and other signaling *** docking results showed that 14-deoxyandrographolide,dehydroandrographolide,licochalcone B,apigenin and naringenin may be the key components of *** in the treatment of AD.[Conclusions]The results suggest that *** can be used to treat Alzheimer s
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