In-depth urinary and exosome proteome profiling analysis identifies novel biomarkers for diabetic kidney disease

作     者：Shichun Du Linhui Zhai Shu Ye Le Wang Muyin Liu Minjia Tan 

作者机构：Department of EndocrinologyXin Hua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Zhongshan Institute for Drug DiscoveryShanghai Institute of Materia MedicaChinese Academy of SciencesGuangdong 528400China Department of CardiologyShanghai Institute of Cardiovascular DiseasesZhongshan HospitalFudan UniversityShanghai 200032China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2023年第66卷第11期

页      面：2587-2603页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China(2020YFE0202200) the National Natural Science Foundation of China(22225702,32071432,32171434,81600702) the Program of Shanghai Academic Research Leader(22XD1420900) the State Key Laboratory of Pharmaceutical Biotechnology,Nanjing University,China(KF-202201) the Innovative Research Team of High-Level Local Universities in Shanghai(SHSMU-ZDCX20212700) the Guangdong High-level New R&D Institute(2019B090904008) the Guangdong High-level Innovative Research Institute(2021B0909050003) 

主　　题：diabetic kidney disease type 2 diabetes mellitus urinary proteome urinary exosome proteome biomarkers 

摘      要：Diabetic kidney disease(DKD)is a major microvascular complication of type 2 diabetes mellitus(T2DM).Monitoring the early diagnostic period and disease progression plays a crucial role in treating *** this study,to comprehensively elucidate the molecular characteristics of urinary proteins and urinary exosome proteins in type 2 DKD,we performed large-scale urinary proteomics(n=144)and urinary exosome proteomics(n=44)analyses on T2DM patients with albuminuria in varying *** dynamics analysis of the urinary and exosome proteomes in our study provides a valuable resource for discovering potential urinary biomarkers in patients with DKD.A series of potential biomarkers,such as SERPINA1 and transferrin(TF),were detected and validated to be used for DKD diagnosis or disease *** results of our study comprehensively elucidated the changes in the urinary proteome and revealed several potential biomarkers reflecting the progression of DKD,which provide a reference for DKD biomarker screening.