Multiphasic bone-ligament-bone integrated scaffold enhances ligamentization and graft-bone integration after anterior cruciate ligament reconstruction

作     者：Xianrui Xie Jiangyu Cai Dan Li Yujie Chen Chunhua Wang Guige Hou Thorsten Steinberg Bernd Rolauffs Mohamed EL-Newehy Hany EL-Hamshary Jia Jiang Xiumei Mo Jinzhong Zhao Jinglei Wu 

作者机构：State Key Laboratory for Modification of Chemical Fibers and Polymer MaterialsShanghai Engineering Research Center of Nano-Biomaterials and Regenerative MedicineCollege of Biological Science and Medical EngineeringDonghua UniversityShanghai201620PR China Department of Sports MedicineShanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200233China School of PharmacyKey Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of ChinaBinzhou Medical UniversityYantai264003China National Engineering Laboratory for Modern SilkSoochow UniversitySuzhou215123China Division of Oral BiotechnologyCenter for Dental MedicineMedical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgHugstetter Str.5579106FreiburgGermany G.E.R.N.Research Center for Tissue ReplacementRegeneration&NeogenesisDepartment of Orthopedics and Trauma SurgeryFaculty of MedicineMedical Center-Albert-Ludwigs-University of Freiburg79085Freiburg im BreisgauGermany Department of ChemistryCollege of ScienceKing Saud UniversityP.O.Box 2455Riyadh11451Saudi Arabia 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2024年第31卷第1期

页      面：178-191页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the Fundamental Research Funds for the Central Universities(2232019A3-07,2232019D3-20) the Science and Technology Commission of Shanghai Municipality(20S31900900,20DZ2254900) the Sino German Science Foundation Research Exchange Center(M􀀀0263) the National Natural Science Foundation of China(82102579,81871753) the Shanghai Rising-Star Program(22QC1401200) the Basic Scientific Research Project of Shanghai Sixth People’s Hospital(ynqn202101) the Opening Project of National Engineering Laboratory for Modern Silk,Soochow University(SDGC2149) the National Key Research and Development Program of China(2018YFC1106200,2018YFC1106202) the Key R&D Program of Shandong province(2019JZZY011104) the Deutsche Forschungsgemeinschaft(German Research Foundation(B.R.:RO 2511/11-1),This project was also supported by Researchers Supporting Project Number(RSP2023R65) King Saud University,Riyadh,Saudi Arabia,and the combined Sino-German Mobility Programme of the National Natural Science Foundation of China(NSFC)/Deutsche Forschungsgemeinschaft(German Research Foundation(B.R.:M􀀀0332) 

主　　题：Electrospun Thermally induced phase separation Integrated scaffold Anterior cruciate ligament reconstruction Graft-bone integration 

摘      要：The escalating prevalence of anterior cruciate ligament(ACL)injuries in sports necessitates innovative strategies for ACL *** this study,we propose a multiphasic bone-ligament-bone(BLB)integrated scaffold as a potential *** BLB scaffold comprised two polylactic acid(PLA)/deferoxamine(DFO)@mesoporous hydroxyapatite(MHA)thermally induced phase separation(TIPS)scaffolds bridged by silk fibroin(SF)/connective tissue growth factor(CTGF)@Poly(L-lactide-co-ε-caprolactone)(PLCL)nanofiber yarn braided *** combination mimics the native architecture of the ACL *** mechanical properties of the BLB scaffolds were determined to be compatible with the human *** vitro experiments demonstrated that CTGF induced the expression of ligament-related genes,while TIPS scaffolds loaded with MHA and DFO enhanced the osteogenic-related gene expression of bone marrow stem cells(BMSCs)and promoted the migration and tubular formation of human umbilical vein endothelial cells(HUVECs).In rabbit models,the BLB scaffold efficiently facilitated ligamentization and graft-bone integration processes by providing bioactive *** double delivery of DFO and calcium ions by the BLB scaffold synergistically promoted bone regeneration,while CTGF improved collagen formation and ligament ***,the findings indicate that the BLB scaffold exhibits substantial promise for ACL *** investigation and advancement of this scaffold may yield enhanced results in the management of ACL injuries.