CIDP: a multi-functional platform for designing CRISPR sgRNAs

作     者：Dong Xu Jin Zhang Xianjia Zhao Yuze Hou Heling Jiang Wenchuang He Xiongfeng Ma Weihua Pan Dong Xu;Jin Zhang;Xianjia Zhao;Yuze Hou;Heling Jiang;Wenchuang He;Xiongfeng Ma;Weihua Pan

作者机构：Shenzhen BranchGuangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518120China State Key Laboratory of Subtropical SilvicultureCollege of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouZhejiang 311300China College of Data ScienceTaiyuan University of TechnologyTaiyuanShanxi 030600China State Key Laboratory of Cotton BiologyInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2023年第10卷第7期

页      面：1-3页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work was supported by National Natural Science Foundation of China(Grant No.32100501) Shenzhen Science and Technology Program(Grant No.RCBS20210609103819020) J.Z.was funded by the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(2021C02070-1) 

主　　题：designing Regular functional 

摘      要：Dear Editor,Compared with traditional technologies affecting gene expression,changing DNA sequences of target genes is one of the most outstanding characters of CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats).Single-guide RNAs(sgRNAs)guiding endonuclease Cas to target sites is a crucial step of CRISPR-Cas system for changing DNA *** ideal sgRNA should only bind to the target ***,similar sequences of non-target sites can also be recognized leading to off-target effects[1].