Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells

作     者：江渝 刘红 彭家和 叶治家 何凤田 董燕麟 刘秉文 

作者机构：Department of Biochemistry & Molecular Biology Third Military Medical University Chongqing 400038 China Department of Hematology Xinqiao Hospital Third Military Medical University Chongqing 400037 China Department of Biochemistry & Molecular Biology West China University of Medical Science Chengdu 610041 China 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：2003年第18卷第2期

页      面：73-76页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：high density lipoprotein human arterial smooth muscle cell oxidative modification cell culture 

摘      要：Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by cultured human arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected, and native HDL (N-HDL) served as control. Oxidative modification of HDL was identified by using agarose gel electrophoresis. Absorbances of conjugated diene (CD) and lipid hydroperoxide (LOOH) were measured with ultraviolet spectrophotometry at 234 and 560 nm respectively, and fluorescence intensity of thiobarbuturic acid reaction substance (TBARS) with fluorescence spectrophotometry at 550 nm emission wavelength with excitation at 515 nm. Results: In comparison with N-HDL, the electrophoretic mobility of SMCs-cocultured HDL was increased, and the contents of CD, LOOH and TBARS HDL were very significantly higher than those of the control HDL (P0.01). Conclusion: Oxidative modification of HDL can be induced by human arterial SMCs.