Effect of Octadecadienoic Acid on Proliferation and Apoptosis of Glioma Cells and Its Mechanism

作     者：Mingren XIE Xia YUAN Shan QI Lei YU Farong YU 

作者机构：School of Public SecurityGansu University of Political Science and LawLanzhou 730070China 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2023年第14卷第4期

页      面：24-26,34页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Gansu Natural Science Foundation(21JR7RA571) Lanzhou Science and Technology Plan Project(2019-1-48) Major Project of Gansu University of Political Science and Law(GZF2021XZD06) 

主　　题：Octadecadienoic acid Glioma cells Inhibition effect Apoptosis 

摘      要：[Objectives] To explore the inhibitory effect of octadecadienoic acid (ODA) on proliferation and apoptosis of glioma cells and its mechanism. [Methods] Cultured human glioma cells (cell density 2×10^(6) cells/L) were divided into three groups: solvent control group (DMSO, 30 μL/L), 5-FU group (10 mg/L) and octadecadienoic acid group (0.3, 0.6, 1.2 mg/L). The toxic effects of ODA on glioma cells were detected by trypan blue and thiazolium blue (MTT). The expression of P53, PI3K, P21, PKB/Akt and caspase-9 protein in glioma cells were detected by enzyme-linked immunosorbent assay (ELISA). [Results] The cell count under optical microscope showed that the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group was significantly higher than that in solvent control group ( P 0.05). The results of MTT showed that compared with the solvent control group, the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group significantly increased ( P 0.01);compared with 5-FU group, the inhibition rate of cell proliferation in high dose ODA group significantly increased ( P 0.01). The results of flow cytometry showed that compared with the solvent control group, the number of cells in G_(0)/G_(1) phase increased significantly ( P 0.05, P 0.01), the number of cells in G_(2)/M phase decreased significantly ( P 0.01) and the apoptosis rate increased significantly ( P 0.01) in the low, medium and high dose ODA groups and 5-FU group;compared with 5-FU group, the number of cells in G_(2)/M phase decreased significantly ( P 0.01) and the apoptosis rate increased significantly ( P 0.01) in ODA group. ELISA testing results showed that the expression levels of P53, P13K and PKB/Akt in low, medium and high dose ODA groups and 5-FU group were significantly lower than those in solvent control group ( P 0.01), and only the expression level