Establishment of a Rat Model of Capillary Leakage Syndrome Induced by Cardiopulmonary Resuscitation After Cardiac Arrest

作     者：Xiao-lei ZHANG Ye CHENG Chun-lin XING Jia-yun YING Xue YANG Xiao-di CAI Guo-ping LU Xiao-lei ZHANG;Ye CHENG;Chun-lin XING;Jia-yun YING;Xue YANG;Xiao-di CAI;Guo-ping LU

作者机构：Department of Pediatric Emergency Medicine and Critical Care MedicineChildren's Hospital of Fudan UniversityShanghai 201102China Department of Pediatric Critical Care MedicineChildren's Hospital of Chongqing Medical UniversityChongqing 400014China 

出 版 物：《当代医学科学(英文)》 (Current Medical Science)

年 卷 期：2023年第43卷第4期

页      面：708-715页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：cardiac arrest cardiopulmonary resuscitation capillary leakage model 

摘      要：Objective:Cardiopulmonary resuscitation(CPR)after cardiac arrest(CA)is one of the main causes of capillary leakage syndrome(CLS).This study aimed to establish a stable CLS model following the CA and cardiopulmonary resuscitation(CA-CPR)model in Sprague-Dawley(SD)***:We conducted a prospective,randomized,animal model *** adult male SD rats were randomly divided into a normal group(group N),a sham operation group(group S),and a cardiopulmonary resuscitation group(group T).The SD rats of the three groups were all inserted with 24-G needles through their left femoral arteries and right femoral *** group S and group T,the endotracheal tube was *** group T,CA induced by asphyxia(AACA)was caused by vecuronium bromide with the endotracheal tube obstructed for 8 min,and the rats were resuscitated with manual chest compression and mechanical *** and postresuscitation measurements,including basic vital signs(BVS),blood gas analysis(BG),routine complete blood count(CBC),wet-to-dry ratio of tissues(W/D),and the HE staining results after 6 h were ***:In group T,the success rate of the CA-CPR model was 60%(18/30),and CLS occurred in 26.6%(8/30)of the *** were no significant differences in the baseline characteristics,including BVS,BG,and CBC,among the three groups(P0.05).Compared with pre-asphyxia,there were significant differences in BVS,CBC,and BG,including temperature,oxygen saturation(SpO_(2)),mean arterial pressure(MAP),central venous pressure(CVP),white blood cell count(WBC),hemoglobin,hematocrit,pH,pCO_(2),pO_(2),SO_(2),lactate(Lac),base excess(BE),and Na+(P0.05)after the return of spontaneous circulation(ROSC)in group *** 6 h after ROSC in group T and at 6 h after surgery in groups N and S,there were significant differences in temperature,heart rate(HR),respiratory rate(RR),SpO_(2),MAP,CVP,WBC,pH,pCO_(2),Na+,and K+among the three groups(P0.05).Compared with the other two groups,the rats in group