Ercao Qinggan decoction regulates apoptosis of hepatocytes in mice with acute liver failure via the TLR4-mediated PI3K/Akt/GSK3βsignal pathway
作者机构：Department of InfectionWenzhou Hospital of Traditional Chinese MedicineWenzhou 325000China
出 版 物：《TMR Integrative Medicine》 (TMR整合医学)
年 卷 期：2023年第7卷第20期
页 面：1-9页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：The National Natural Science Foundation of Zhejiang Provincial(No.LGF21H270001) the Association of Integrated Chinese and Western Medicine Research Fund Project of Zhejiang Provincial(No.2019LY013) the Basic Research Project of Wenzhou Science and Technology Bureau(No.Y20210149)supported this study
主 题：acute liver failure Ercao Qinggan decoction lipopolysaccharide HL-7702 TLR4
摘 要：Background:To clarify the inhibitory effect of Ercao Qinggan decoction(EQD)on acute liver failure(ALF)and its related ***:HL-7702 hepatocytes were pretreated with TLR4 inhibitor CLI-095,glycogen synthase kinase 3β(GSK3β)inhibitor LiCl and different doses of EQD for 2 hours,and lipopolysaccharide(LPS)(10μg/mL)for 24 *** apoptosis,TNF-αand IL-6 and GSK3βwere detected by flow cytometry,immunofluorescence,quantitative polymerase chain *** mice were gavaged with different concentrations of EQD for 12 days,ALF mouse models were established intraperitoneal injection of D-Gal/*** 24 hours,the mice were euthanized and the liver tissue was stained with hematoxylin and *** cell apoptosis,the serum levels of aspartate aminotransferase,alanine aminotransferase,TNF-αand IL-βwere detected by terminal transferase-mediated dUTP nick end-labelling,enzyme linked immunosorbent assay,quantitative polymerase chain reaction,and Western blotting,*** methods were also used to test the mRNA expression of Bax,Bcl-2 and the protein expression of GSK3β,p-Akt/Akt in ***:CLI-095,LiCl,and EQD significantly inhibited apoptosis induced by LPS,the mRNA expression of IL-6,TNF-αand the nuclear translocation of GSK3βin HL-7702 *** dose-dependently inhibited hepatocyte apoptosis,the serum concentration of aspartate aminotransferase and ALT,the expression of TNF-αand IL-β,the ratio of p-GSK3β/GSK3β,p-Akt/Akt in alanine aminotransferase ***:EQD can inhibit hepatocyte apoptosis in ALF mice through regulating TLR4/PI3K/Akt/GSK3βsignaling pathway.
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