Scaffold protein RACK1A positively regulates leaf senescence by coordinating the EIN3-miR164-ORE1 transcriptional cascade in Arabidopsis

作     者：Jan Masood Wei Zhu Yajuan Fu Zhiyong Li Yeling Zhou Dong Zhang Huihui Han Yan Yan Xing Wen Hongwei Guo Jiansheng Liang Jan Masood;Wei Zhu;Yajuan Fu;Zhiyong Li;Yeling Zhou;Dong Zhang;Huihui Han;Yan Yan;Xing Wen;Hongwei Guo;Jiansheng Liang

作者机构：Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education InstitutesInstitute of Plant and Food SciencesDepartment of BiologySchool of Life SciencesSouthern University of Science and Technology518055ShenzhenChina 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第7期

页      面：1703-1716页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by grants from the National Natural Science Foundation of China (32070292 to J.L.) Shenzhen Science and Technology Program (KQTD2019 0929173906742 to J.L.) The Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes (2019KSYS006 to J.L.) Scientific Research Funding for postdoctoral researchers staying at Shenzhen (K19227561, K21227504 to W.Z.)。 

主　　题：Arabidopsis ethylene signaling leaf senescence RACK1 scaffold protein transcriptional cascade 

摘      要：Plants have adopted versatile scaffold proteins to facilitate the crosstalk between multiple signaling pathways. Leaf senescence is a well-programmed developmental stage that is coordinated by various external and internal signals. However,the functions of plant scaffold proteins in response to senescence signals are not well understood. Here, we report that the scaffold protein RACK1A(RECEPTOR FOR ACTIVATED C KINASE1A) participates in leaf senescence mediated by ethylene signaling via the coordination of the EIN3-miR164-ORE1 transcriptional regulatory cascade. RACK1A is a novel positive regulator of ethylene-mediated leaf senescence. The rack1a mutant exhibits delayed leaf senescence, while transgenic lines overexpressing RACK1A display early leaf senescence. Moreover, RACK1A promotes EIN3(ETHYLENE INSENSITIVE 3) protein accumulation, and directly interacts with EIN3 to enhance its DNA-binding activity. Together, they then associate with the miR164 promoter to inhibit its transcription, leading to the release of the inhibition on downstream ORE1(ORESARA 1) transcription and the promotion of leaf senescence.This study reveals a mechanistic framework by which RACK1A promotes leaf senescence via the EIN3-miR164-ORE1 transcriptional cascade, and provides a paradigm for how scaffold proteins finely tune phytohormone signaling to control plant development.