A tetrahedral framework nucleic acid based multifunctional nanocapsule for tumor prophylactic mRNA vaccination

作     者：Yuhao Liu Songhang Li Shiyu Lin Sirong Shi Taoran Tian Bowen Zhang Tao Zhang Yunfeng Lin Yuhao Liu;Songhang Li;Shiyu Lin;Sirong Shi;Taoran Tian;Bowen Zhang;Tao Zhang;Yunfeng Lin

作者机构：State Key Laboratory of Oral DiseasesNational Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan University Chengdu 610041China Key Laboratory of Oral Biomedical Research of Zhejiang ProvinceZhejiang UniversityHangzhou 31000China Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhou 510055China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2023年第34卷第7期

页      面：169-175页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 070205[理学-凝聚态物理] 0703[理学-化学] 10[医学] 0702[理学-物理学] 

基　　金：supported by National Key R&D Program of China (No. 2019YFA0110600) National Natural Science Foundation of China (No. 81970916) Sichuan Province Youth Science and Technology Innovation Team (No. 2022JDTD0021) Research Funding from West China School/Hospital of Stomatology Sichuan University (No. RCDWJS2021-20) China Postdoctoral Science Foundation (No. 2022TQ0381)。 

主　　题：Tetrahedral framework nucleic acid(tFNA) RNA nanovaccine Cytosine-phosphate-guanine(CpG)motifs Murineβ-defensin 2(mDF2β) Tumor immunotherapy 

摘      要：Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.