Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants
作者机构：State Key Laboratory of Materials-Oriented Chemical EngineeringSchool of Pharmaceutical SciencesNanjing Tech UniversityNanjing 210009China
出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)
年 卷 期：2023年第17卷第7期
页 面：867-879页
核心收录：
学科分类：07[理学] 070303[理学-有机化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学]
基 金：This work was supported by the National Natural Science Foundation of China(Grant No.22178174) the National Key R&D Program of China(Grant No.2021YFC2103802) the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(Grant No.XTC2206).
主 题：polydopamine pollutant removal thermodynamic phenolic pollutants immobilized laccase
摘 要：Polydopamine-functionalized nanosilica was synthesized using an inexpensive and easily obtainable raw material,mild reaction conditions,and simple operation.Subsequently,a flexible spacer arm was introduced by using dialdehyde starch as a cross-linking agent to bind with laccase.A high loading amount(77.8 mg∙g^(‒1))and activity retention(75.5%)could be achieved under the optimum immobilization conditions.Thermodynamic parameters showed that the immobilized laccase had a lower thermal deactivation rate constant and longer half-life.The enhancement of thermodynamic parameters indicated that the immobilized laccase had better thermal stability than free laccase.The residual activity of immobilized laccase remained at about 50.0%after 30 days,which was 4.0 times that of free laccase.Immobilized laccase demonstrated excellent removal of phenolic pollutants(2,4-dichlorophenol,bisphenol A,phenol,and 4-chlorophenol)and perfect reusability with 70% removal efficiency retention for 2,4-dichlorophenol after seven cycles.These results suggested that immobilized laccase possessed great reusability,improved thermal stability,and excellent storage stability.Organic–inorganic nanomaterials have a good application prospect for laccase immobilization,and the immobilized laccase of this work may provide a practical application for the removal of phenolic pollutants.
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