Determination of selectivity of HPLC systems by correspondence factor analysis

作     者：Yuan Wang Jun Yang Xin Lu Guo Wang Xu 

作者机构：National Chromatographic Research ＆ Analysis Center Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2007年第18卷第5期

页      面：565-568页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：This study has been supported by the National Natural Science Foundation of China (No. 20675082) the Foundation for Distinguished Young Scholars from National Natural Science Foundation of China (No. 20425516) the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-SW-329, KGCX2-SW-213) 

主　　题：CFA HILIC Liquid chromatography 

摘      要：Correspondence factor analysis （CFA） was employed to study the selectivity of 14 HPLC systems. The tested LC systems were classified as reversed-phase （RP）, ion-exchange （IE） and hydrophilic interaction chromatography （HILIC） modes. It was found that the retentions of the hydrophilic solutes on HILIC column were significantly influenced by the second-order effects besides their hydrophilic properties. Organic modifiers and residue silanol groups on silica surface both participated in retention. HypersilTM amino column performed separation in the HILIC mode at appropriate conditions, and its retention mechanism was more similar to that of HILIC silica column than that of HILIC column coating poly（aspartamide） groups.