The Anterior Insula and its Projection to the Prelimbic Cortex are Involved in the Regulation of 5-HT-Induced Itch

作     者：Juan Yao Xuan Li Guang-Yan Wu Bing Wu Jun-Hui Long Pu-Jun Wang Shu-Lei Liu Jie Gao Jian-Feng Sui 

作者机构：Experimental Center of Basic MedicineArmy Medical UniversityChongqing400038China Department of PhysiologyCollege of Basic Medical SciencesArmy Medical UniversityChongqing400038China Department of DermatologyThe 958th Army Hospital of the People’s Liberation ArmyChongqing400020China State Key Laboratory of TraumaBurns and Combined InjuryArmy Medical Centre of the PLAInstitute of Surgery ResearchDaping HospitalArmy Medical UniversityChongqing400042China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2023年第39卷第12期

页      面：1807-1822页

核心收录：

学科分类：0402[教育学-心理学(可授教育学、理学学位）] 040203[教育学-应用心理学] 04[教育学] 

基　　金：supported by the National Natural Science Foundation of China(82271542) the Natural Science Foundation of Chongqing(cstc2020jcyj-msxm X0391 and cstc2019jcyj-msxm X0269) the Chongqing Medical Scientific Research Project(Joint project of Chongqing Health Commission and Science and Technology Bureau)(2023ZDXM001) 

主　　题：Itch-Insular cortex Prelimbic cortex Conditioned place aversion Pharmacogenetic inhibition 

摘      要：Itch is an unpleasant sensation that urges people and animals to *** studies on itch have yielded extensive correlations with diverse cortical and subcortical regions,including the insular ***,the role and functional specificity of the insular cortex(IC)and its subdivisions in itch mediation remains ***,we demonstrated by immunohistochemistry and fiber photometry tests,that neurons in both the anterior insular cortex(AIC)and the posterior insular cortex(PIC)are activated during acute itch *** experiments revealed that nonselective inhibition of global AIC neurons,or selective inhibition of the activity of glutaminergic neurons in the AIC,reduced the scratching behaviors induced by intradermal injection of 5-hydroxytryptamine(5-HT),but not those induced by compound 48/***,both nonselective inhibition of global PIC neurons and selective inhibition of glutaminergic neurons in the PIC failed to affect the itching-scratching behaviors induced by either 5-HT or compound 48/*** addition,pharmacogenetic inhibition of AIC glutaminergic neurons effectively blocked itch-associated conditioned place aversion behavior,and inhibition of AIC glutaminergic neurons projecting to the prelimbic cortex significantly suppressed 5-HT-evoked *** findings provide preliminary evidence that the AIC is involved,at least partially via aversive emotion mediation,in the regulation of 5-HT-,but not compound 48/80-induced itch.