Hedgehog signaling pathway regulated the target genes for adipogenesis in silkworm Bombyx moil
Hedgehog signaling pathway regulated the target genes for adipogenesis in silkworm Bombyx moil作者机构：Key Laboratory of Animal Virology of Ministry of Agriculture College of Animal Sciences Zhejiang University Hangzhou 310058 China
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2015年第22卷第5期
页 面：587-596页
核心收录：
学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：国家自然科学基金 国家973计划 the Key project of Zhejiang Government the Science and Technology Innovation Team of Zhejiang Province Chinese Universities Scientific Fund
主 题：adipocyte differentiation adipogenesis Bombyx mori Hedgehog (Hh) signaling pathway silkworm
摘 要：Hedgehog （Hh） signals regulate invertebrate and vertebrate development, yet the role of the pathway in adipose development remains poorly understood. In this report, we found that Hh pathway components are expressed in the fat body of silkworm larvae. Functional analysis of these components in a BmN cell line model revealed that activa- tion of the Hh gene stimulated transcription of Hh pathway components, but inhibited the expression of the adipose marker gene AP2. Conversely, specific RNA interference- mediated knockdown of Hh resulted in increased AP2 expression. This further showed the regulation of Hh signal on the adipose marker gene. In silkworm larval models, enhanced adipocyte differentiation and an increase in adipocyte cell size were observed in silkworms that had been treated with a specific Hh signaling pathway antagonist, cyclopamine. The fat-body-specific Hh blockade tests were consistent with Hh signaling inhibiting silkworm adipogenesis. Our results indicate that the role of Hh signaling in inhibiting fat formation is conserved in vertebrates and invertebrates.
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