CATase-immobilized hydrogel platform molded by photo-enzyme coupling polymerization for effectively preventing postoperative abdominal adhesion

作     者：Min Hu Mingyue Shi Xia Wang Yujing Tang Dingze Zhou Kai Pan Qigang Wang 

作者机构：School of Chemical Science and EngineeringTongji UniversityShanghai 200092China Translational Research Institute of Brain and Brain-Like IntelligenceShanghai Fourth People’s HospitalSchool of MedicineTongji UniversityShanghai 200434China 

出 版 物：《中国科学:化学英文版》 (SCIENCE CHINA Chemistry)

年 卷 期：2023年第66卷第9期

页      面：2664-2675页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：supported by the National Science Fund for Distinguished Young Scholars(52125305) the National Natural Science Foundation of China(52173289,52273147) the Key Project of the First Demonstration Project(Artificial intelligence)of Interdisciplinary Joint Research of Tongji University(ZD-11-202151) 

主　　题：photo-enzyme coupling polymerization enzyme immobilization antioxidative anti-inflammatory prevention abdominal adhesion 

摘      要：Redox homeostasis,which is regulated by enzymes acting as regulatory valves,is crucial for maintaining the proper functioning of biomolecules and a stable microenvironment for physiological processes by modulating the homeostasis of reactive oxygen species(ROS).Antioxidant enzymes in biocatalysis are used in the prevention or treatment of oxidative stress-related disease by counteracting the harmful effects of ***,designing a system that can efficiently immobilize antioxidant enzymes with high catalytic activity and stability is still *** by photo-biocatalysis,a novel and effective catalase(CATase)-immobilized hydrogel platform has been developed by the proposed photo-enzymatic coupled radical polymerization strategy of the visible light coupling with the porphyrin-centered *** higher catalytic stability and activity can therefore be achieved due to the preferential polymerization of CATase-immobilized hydrogel platform with a favorable three-dimensional network of enhanced coupling efficacy between light and *** mechanisms of free radical-initiated polymerization as well as the antioxidant cycle in the photo-CATase coupling system have been ***,the CATase-immobilized hydrogel platform affords an unprecedented antioxidant ability to scavenge ROS and provide an effective cellular protection mechanism against external oxidative ***,the CATase-immobilized hydrogel platform can effectively prevent peritoneal adhesion by reducing the expression of inflammatory ***,the novel CATase-immobilized hydrogel platform is a potential candidate for physical barriers that effectively prevent postoperative adhesion formation,offering a new anti-adhesion strategy for clinical applications.