Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing

作     者：Anamaria Balic Dilara Perver Pierfrancesco Pagella Hubert Rehrauer Bernd Stadlinger Andreas E.Moor Viola Vogel Thimios A.Mitsiadis Anamaria Balic;Dilara Perver;Pierfrancesco Pagella;Hubert Rehrauer;Bernd Stadlinger;Andreas E.Moor;Viola Vogel;Thimios A.Mitsiadis

作者机构：Orofacial Development and RegenerationInstitute of Oral BiologyCentre of Dental MedicineUniversity of ZurichZurichSwitzerland Department of Health Sciences and TechnologyInstitute of Translational MedicineETH ZurichZurichSwitzerland Functional Genomics Center ZurichETH Zurich and University of ZurichZurichSwitzerland Clinic of Cranio-Maxillofacial and Oral SurgeryUniversity of ZurichZurichSwitzerland Department of Biosystems Science and EngineeringETH ZurichBaselSwitzerland 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志(英文版))

年 卷 期：2023年第15卷第3期

页      面：450-461页

核心收录：

学科分类：1003[医学-口腔医学] 100301[医学-口腔基础医学] 10[医学] 

基　　金：Eidgenössische Technische Hochschule Zürich, ETH Universität Zürich, UZH Functional Genomics Center Zurich, FGCZ 

主　　题：organs pulp matrix 

摘      要：Carious lesions are bacteria-caused destructions of the mineralised dental tissues,marked by the simultaneous activation of immune responses and regenerative events within the soft dental pulp *** major molecular players in tooth decay have been uncovered during the past years,a detailed map of the molecular and cellular landscape of the diseased pulp is still *** this study we used single-cell RNA sequencing analysis,supplemented with immunostaining,to generate a comprehensive single-cell atlas of the pulp of carious human *** data demonstrated modifications in the various cell clusters within the pulp of carious teeth,such as immune cells,mesenchymal stem cells(MSC)and fibroblasts,when compared to the pulp of healthy human *** immune response in the carious pulp tissue is accompanied by specific changes in the fibroblast and MSC *** changes include the upregulation of genes encoding extracellular matrix(ECM)components,including COL1A1 and Fibronectin(FN1),and the enrichment of the fibroblast cluster with *** incremental changes in the ECM composition of carious pulp tissues were further confirmed by immunostaining *** of the Fibronectin fibres under mechanical strain conditions showed a significant tension reduction in carious pulp tissues,compared to the healthy *** present data demonstrate molecular,cellular and biomechanical alterations in the pulp of human carious teeth,indicative of extensive ECM remodelling,reminiscent of fibrosis observed in other *** comprehensive atlas of carious human teeth can facilitate future studies of dental pathologies and enable comparative analyses across diseased organs.