CRISPR/Cas systems for the detection of nucleic acid and nonnucleic acid targets
作者机构：State Key Laboratory of Oncogenes and Related GenesShanghai Cancer InstituteRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200032China Central LaboratoryRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Micro-Nano Research and Diagnosis CenterRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2023年第16卷第7期
页 面：9940-9953页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
基 金：This work was financially supported by the National Natural Science Foundation of China(Nos.81771968 and 22204104) the Shanghai Sailing Program(No.21YF1444900) the Shanghai Municipal Natural Science Foundation(No.22ZR1459600) the Medical-Engineering Joint Funds from the Shanghai Jiao Tong University(Nos.YG2023ZD07 and YG2021QN23) the Foundation of Shanghai Municipal Health Commission(No.2022JC002) the Innovative Research Team of High-Level Local Universities in Shanghai,China.
主 题：clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) detection nucleic acid targets non-nucleic acid targets
摘 要：Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)systems are becoming powerful tools for disease biomarkers detection.Due to the specific recognition,cis-cleavage and nonspecific trans-cleavage capabilities,CRISPR/Cas systems have implemented the detection of nucleic acid targets(DNA and RNA)as well as non-nucleic acid targets(e.g.,proteins,exosomes,cells,and small molecules).In this review,we first summarize the principles and characteristics of various CRISPR/Cas systems,including CRISPR/Cas9,Cas12,Cas13 and Cas14 systems.Then,various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically.Finally,the prospects and challenges of their applications in biosensing are discussed.
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