Non-invasive imaging of pathological scars using a portable handheld two-photon microscope
Non-invasive imaging of pathological scars using a portable handheld two-photon microscope作者机构:Graduate SchoolChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100730China Department of DermatologyChina-Japan Friendship HospitalBeijing 100029China College of EngineeringPeking UniversityBeijing 100871China School of Chemical Biology and BiotechnologyState Key Laboratory of Chemical OncogenomicsPeking University Shenzhen Graduate SchoolShenzhenGuangdong 518055China Department of DermatologyThe First Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006China Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China College of Future TechnologyPeking UniversityBeijing 100871China Beijing Transcend Vivoscope BiotechBeijing 100085China Department of DermatologyThe First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230001China Institute of DermatologyAnhui Medical UniversityHefeiAnhui 230001China School of ElectronicsPeking UniversityBeijing 100871China
出 版 物:《Chinese Medical Journal》 (中华医学杂志(英文版))
年 卷 期:2024年第137卷第3期
页 面:329-337页
核心收录:
学科分类:1002[医学-临床医学] 100210[医学-外科学(含:普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金:supported by grants from Beijing Municipal Science and Technology Commission Medicine Collaborative Science and Technology Innovation Research Project(No.Z191100007719001) To Establish a Database and Study the Imaging Features of Common Skin Diseases based on Two-photon Imaging Technology(No.SK2021090379-1)
主 题:Scars Two-photon microscope Two-photon excitation fluorescence Second harmonic generation microscopy
摘 要:Background:Pathological scars are a disorder that can lead to various cosmetic,psychological,and functional problems,and no effective assessment methods are currently *** and treatment of pathological scars are based on cutaneous manifestations.A two-photon microscope(TPM)with the potential for real-time non-invasive assessment may help determine the under-surface pathophysiological conditions in *** study used a portable handheld TPM to image epidermal cells and dermal collagen structures in pathological scars and normal skin in vivo to evaluate the effectiveness of treatment in scar ***:Fifteen patients with pathological scars and three healthy controls were *** was performed using a portable handheld *** indexes were extracted from two dimensional(2D)and three dimensional(3D)perspectives,including collagen depth,dermo-epidermal junction(DEJ)contour ratio,thickness,orientation,and occupation(proportion of collagen fibers in the field of view)of *** depth-dependent indexes were computed through the 3D second harmonic generation image and three morphology-related indexes from the 2D *** assessed index differences between scar and normal skin and changes before and after ***:Pathological scars and normal skin differed markedly regarding the epidermal morphological structure and the spectral characteristics of collagen *** indexes were employed to distinguish between normal skin and scar *** significant differences were found in average depth(t=9.917,P0.001),thickness(t=4.037,P0.001),occupation(t=2.169,P0.050),orientation of collagen(t=3.669,P0.001),and the DEJ contour ratio(t=5.105,P0.001).Conclusions:Use of portable handheld TPM can distinguish collagen from skin tissues;thus,it is more suitable for scar imaging than reflectance confocal ***,a TPM may be an auxiliary tool for scar treatment selection and assessing treatment efficacy.