Lotus root polysaccharide inhibits the growth of ovarian cancer cells by blocking the cell cycle
作者机构：College of Veterinary MedicineQingdao Agricultural UniversityQingdao 266100China Instrumental Analysis Center of Qingdao Agricultural UniversityQingdao 266100China
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2023年第8卷第8期
页 面：18-24页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：lotus root polysaccharide ovarian cancer SKOV3 RNA-seq
摘 要：Background:Lotus root polysaccharide is a natural *** a new anticancer drug,it has anti-proliferation and pro-apoptotic effects in a variety of tumour cells,but its effect on ovarian cancer is not *** study,we attempted to elucidate the role and mechanism of lotus root polysaccharide in SKOV3 ***:In this study,the effect of lotus root polysaccharide on mRNA of SKVO3 cells was analyzed by RNA-seq,and verified by Western blot,flow cytometry,fluorescence detection and other ***:The results showed that lotus root polysaccharide could inhibit the proliferation of ovarian cancer ***,a change in gene expression was found by *** the mRNA(differentially expressed mRNA)with these differences,significant changes in the cell cycle were found by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment ***,the proportion of cells in S phase decreases and G2/M phase increases,as seen with propidium iodide *** Set Enrichment Analysis showed inhibition of the cell cycle,and the gene and protein expression of CDK1,CCNA1 and CCNB1 were ***:Our results show that lotus root polysaccharide can inhibit the growth of SKOV3 cells in vitro by blocking the cell cycle at the G2/M phase,which reveals the potential of lotus root polysaccharide in the treatment of ovarian cancer.
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