Synthesis of RNA-Amphiphiles via Atom Transfer Radical Polymerization in the Organic Phase

作     者：Jaepil Jeong Grzegorz Szczepaniak Subha R.Das Krzysztof Matyjaszewski 

作者机构：Department of ChemistryCarnegie Mellon UniversityPittsburghPennsylvania 15213United States Center for Nucleic Acids Science&TechnologyCarnegie Mellon UniversityPittsburghPennsylvania 15213United States University of WarsawFaculty of Chemistry02-093 WarsawPoland 

出 版 物：《Precision Chemistry》 (精准化学（英文）)

年 卷 期：2023年第1卷第5期

页      面：326-331页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：NSF DMR 2202747 and DTRA grant HDTRA1-20-1-0014.G.S.gratefully acknowledges the Polish National Agency for Academic Exchange(BPN/PPO/2022/1/00027)for financial support 

主　　题：ATRP RNA RNA-amphiphiles nucleic acid block copolymer RNA−polymer conjugate TBDMS SBiB phosphoramidite solid-phase RNA synthesis 

摘      要：The combination of hydrophobic polymers with nucleic acids is a fascinating way to engineer the self-assembly behavior of nucleic acids into diverse nanostructures such as micelles,vesicles,nanosheets,and *** we developed a robust route to synthesize a RNA macroinitiator with protecting groups on the 2′-hydroxyl groups in the solid phase using an oligonucleotide *** protecting groups successfully solubilized the RNA macroinitiator,enabling atom transfer radical polymerization(ATRP)of hydrophobic *** a result,the RNA−polymer hybrids obtained by ATRP exhibited enhanced chemical stability by suppressing *** addition,we demonstrated evidence of controlled polymerization behavior as well as control over the molecular weight of the hydrophobic polymers grown from *** envision that this methodology will expand the field of RNA−polymer conjugates while vastly enhancing the possibility to alter and engineer the properties of RNA-based polymeric materials.