In Vitro Picloram-lnduced Somatic Embryogenesis from Leaflets of Cherry （Prunus incisa Thunb,）
In Vitro Picloram-lnduced Somatic Embryogenesis from Leaflets of Cherry （Prunus incisa Thunb,）作者机构：Institut National Agronomique de Tunisie Cite El Mahrajene Tunis 1082 Tunisie Departement Biotechnologie et Physiologie Vegetale Institut National de la Recherche Agronomique de Tunisie Rue Hedi KarrayAriana 2080 Tunisie Unite ＂Genie Biologique ＂ Departement Sciences du Vivant Centre Wallon de Recherches Agronomiques Chaussee de Charleroi234 Gembloux 5030 Belgique
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2011年第5卷第11期
页 面：913-920页
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 090201[农学-果树学] 090102[农学-作物遗传育种]
主 题：Auxin picloram somatic embryogenesis leaf explants Prunus incisa
摘 要：Cherry regeneration via somatic embryogenesis is a powerful tool to breeding. In this way, the embryogenic capacity of Prunus incisa specie has been tested from leaves under different interactions of picloram concentrations and darkness exposures. Induction culture was achieved on MS medium supplemented with picloram concentrations at 0.5, 1 and 1.5 mg.L1 and submitted to 10, 20, 30 and 40 days of darkness. The best rate of embryogenic leaves was obtained with the interaction of 30 days darkness exposure＊ 1 mg.L^-1 picloram. According to their age, leaves were differently reacted to somatic embryogenesis; indeed, the 2nd expanded leaf from the apex was the most embryogenic one. Concerning the effect of additional auxin to picloram （1 mg·L^-1）, IAA at 0.1 mg·L^-1 and IBA at 0.1 mg·L^-1 gave significantly higher induction rates than all other concentrations, but regenerating somatic embryos showed some teratological abnormalities probably due to seconda;y embryogenesis. At the opposite, NAA at 0.5 mg·L^-1 didn＇t improve embryogenic rate but affected positively embryo development. Furthermore, embryogenesis preferentially took place on the basal part of leaf. Satisfactory rates of somatic embryogenesis are obtained but further improvement remains possible.
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