Protective effect of icariin on Aβ25-35-induced HT22 cell injury by ameliorating glycolytic dysfunction through activating Wnt/β-catenin signaling pathway
作者机构：Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical University
出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)
年 卷 期：2023年第37卷第7期
页 面：513-514页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：National Natural Science Foundation of China (82060727) National Natural Science Foundation of China (81660599) Key Projects of Basic Research Program of the Science and Technology Department of Guizhou Province (ZK058)
摘 要：OBJECTIVE To investigate whether icari in (ICA) plays a neuroprotective role by improving glyco lytic function through activating Wnt/β-catenin signaling *** HT22 cells were treated with Aβ25-35for 24 h to establish AD cell model,ICA was added in 2 h before Aβ25-35and the DKK1(a specific inhibitor of the Wnt signaling pathway) was added in 0.5 h before ICA Pharmacodynamic study:HT22 cells were divided into control group,ICA group (ICA 10μmol·L-1),model group(Aβ25-3520μmol·L-1),model+ICA group (Aβ25-3520μmol·L-1+ICA 2.5,5,10μmol·L-1);Mechanism study:HT22 cells were divided into control group,model group,Aβ25-35+ICA10μmol·L-1group,Aβ25-35+DKK1 group,Aβ25-35+DKK1+ICA *** cell viability was detected by MTT assay and the cell morphology was obtained by microscope the lactate content was detected by lactate assay,the ATP content was measured with the chemiluminescence method,the expression levels of HK1,PKM1 and the pro tein expression of molecules related to the Wnt/β-catenin signaling pathway (Wnt3a,GSK3β,pGSK3β Try216pGSK3β Ser9,β-catenin,pβ-catenin Ser33/37 Thr41Active β-catenin and nuclear β-catenin) was assayed by Western *** nuclear translocation of β-catenin was observed by immunofluorescent *** Compared with the control group,the viability of cells in the model group was reduced,the morphology of cells was significantly damaged,the ATP content and lactate content were significantly decreased,and the glycolytic key enzymes:the protein levels of HK1,PKM1 and the protein levels of Wnt3a,pGSK3β Ser9,active β-catenin and nuclear β-catenin were significantly reduced,and the phosphorylation levels of β-catenin Ser33/37 Thr41 were significantly *** with the model group the cell morphology was significantly improved and the viability was significantly increased,the ATP and lactate content were significantly increased,the expressions of HK1,PKM1 and Wnt3a,pGSK3β Ser9,active β-catenin and nuclear β-catenin protein were
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