ENTPD1-AS1-miR-144-3p-mediated high expression of COL5A2 correlates with poor prognosis and macrophage infiltration in gastric cancer
作者机构：Department of Laboratory MedicineThe Eighth Affiliated HospitalSun Yat-sen UniversityShenzhen 518033Guangdong ProvinceChina Department of Clinical LaboratoryThe General Hospital of Western Theater CommandChengdu 610000Sichuan ProvinceChina
出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)
年 卷 期：2023年第15卷第7期
页 面：1182-1199页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by National Natural Science Foundation of China,No.81971489 Natural Science Foundation of Guangdong Province,No.2022A1515011122
主 题：COL5A2 Noncoding RNAs Macrophage infiltration Prognosis Gastric cancer
摘 要：BACKGROUND Gastric cancer(GC)is a malignant tumor with high morbidity and *** of COL5A2 is significantly elevated in *** expression of noncoding RNAs(ncRNAs)have been found in GC,including microRNA(miRNA)and long noncoding RNA(lncRNA).Competing endogenous RNA network plays an important regulatory role in ***,its specific regulatory mechanism has not been *** To gain insight into the ncRNA regulatory mechanism and immune microenvironment related to COL5A2 in *** RNA sequencing data and clinical information from The Cancer Genome Atlas data portal were used to analyze the expressions of COL5A2,miRNA and lncRNA related to the prognosis of *** regression analysis and Kyoto Encyclopedia of Genes and Genomes analysis were performed to assess the risk factors and relevant function of *** was used to predict the interaction of miRNA–lncRNA or miRNA–mRNA in *** relationship between COL5A2,miR-144-3p and ENTPD1-AS1 were verified by dual luciferase reporter *** association of COL5A2 with immune cell infiltration were analyzed using the Tumor Immune Estimation Resource database and single sample gene set enrichment *** expression of COL5A2 and macrophages in paired GC tissues were detected by immunohistochemical *** We verified that the upregulation of COL5A2 expression was associated with the prognosis of GC and was an independent risk factor for ***-144-3p was downregulated and correlated with the prognosis of ***-144-3p regulated the expression of COL5A2 through direct interaction with ***1-AS1 was elevated in GC and competitively bound to miR-144-3p,thus inhibiting the expression of ***1-AS1 enhanced the expression of COL5A2 through sponging *** to paired normal tissue,COL5A2 expression was upregulated at the protein level,especially in the middle and late stages of *** high expression of COL5A2 was positively linked to macrophage in
维普期刊数据库