Neural lineage differentiation of human pluripotent stem cells:Advances in disease modeling

作     者：Yuan-Wei Yan Eddie S Qian Lauren E Woodard Julie Bejoy 

作者机构：Waisman CenterUniversity of Wisconsin-MadisonMadisonWI 53705United States Nephrology and HypertensionVanderbilt University Medical CenterNashvilleTN 37232United States Department of MedicineVanderbilt University Medical CenterNashvilleTN 37232United States Department of Veterans AffairsTennessee Valley Healthcare SystemNashvilleTN 37232United States Biomedical EngineeringVanderbilt UniversityNashvilleTN 37232United States 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2023年第15卷第6期

页      面：530-545页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：CTSA Award from the National Center for Advancing Translational Sciences to the Vanderbilt Institute for Clinical and Translational Research,No.UL1 TR002243 A Pilot and Feasibility Award from the NIDDK to the Vanderbilt Diabetes Research and Training Center,No.DK020593 a VA MERIT Award,No.BX004845. 

主　　题：Induced pluripotent stem cells Astrocytes Oligodendrocytes Microglia Brain organoids Assembloids 

摘      要：Brain diseases affect 1 in 6 people worldwide.These diseases range from acute neurological conditions such as stroke to chronic neurodegenerative disorders such as Alzheimer’s disease.Recent advancements in tissue-engineered brain disease models have overcome many of the different shortcomings associated with the various animal models,tissue culture models,and epidemiologic patient data that are commonly used to study brain disease.One innovative method by which to model human neurological disease is via the directed differentiation of human pluripotent stem cells(hPSCs)to neural lineages including neurons,astrocytes,and oligodendrocytes.Three-dimensional models such as brain organoids have also been derived from hPSCs,offering more physiological relevance due to their incorporation of various cell types.As such,brain organoids can better model the pathophysiology of neural diseases observed in patients.In this review,we will emphasize recent developments in hPSC-based tissue culture models of neurological disorders and how they are being used to create neural disease models.