Mutagenesis of odorant coreceptor Orco reveals the distinct role of olfaction between sexes in Spodoptera frugiperda

作     者：CAO Song SUN Dong-dong LIU Yang YANG Qing WANG Gui-rong CAO Song;SUN Dong-dong;LIU Yang;YANG Qing;WANG Gui-rong

作者机构：Shenzhen BranchGuangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120P.R.China State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第7期

页      面：2162-2172页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 

基　　金：the National Natural Science Foundation of China(32130089) the China Postdoctoral Science Foundation(2020M680785) the Shenzhen Science and Technology Program,China(KQTD20180411143628272) the Central Public-interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202108) the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District,China(PT202101-02) the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP) 

主　　题：Spodoptera frugiperda Orco CRISPR/Cas9 EAG olfactory behavior 

摘      要：Odorant receptor(OR)is crucial for insects to detect and recognize external chemical cues closely related to their *** insect OR forms a heteromeric complex composed of a ligand-specific receptor and a ubiquitously odorant receptor coreceptor(Orco).This study used the CRISPR/Cas9 technique to knock out(KO)Orco and reveal its essential role in acting on OR-meditated olfactory behavior in a critical invasive agricultural pest,the fall armyworm(FAW),Spodoptera ***(EAG)results suggested that the Orco mutants of both male and female moths severely reduced their electrophysiological responses to the eight tested plant volatiles and two sex ***,the Orco gene played distinct roles in mating behavior between sexes:the mating behavior was fully disrupted in mutated males but not in mutated *** oviposition result indicated that the Orco KO females displayed reduced egg laying by 24.1%compared with the mated wild type(WT)***,these results strongly suggest that Orco is an excellent target for disrupting FAW’s normal behavior and provides a feasible pest control approach.