Construction of neuron specific vector of human antisense noggin gene expression

作     者：Shengnian Zhou Chengshan Li Xiansen Wei Liqing Liu Zhengda Zhang 

作者机构：Department of Neurology Qilu Hospital of Shandong University Jinan 250012 Shandong Province China Department of Cadre Medicine China-Japan Friendship Hospital Beijing 100029 China Department of Basic Medicine Shandong University of Traditional Chinese Medicine Jinan 250355 Shandong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第15期

页      面：1171-1174页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071008[理学-发育生物学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the Science and Technology Development Program of Shandong Province,No.2007GG10002017 the Natural Science Foundation of Shandong Province,No.Y2008C63 

主　　题：noggin alphal-tubulin expression vector antisense nucleic acids nerve cells neural regeneration 

摘      要：Thenoggin gene is present in the central nervous system in embryonic and postnatal mammals, and plays an important role in maintaining nervous system development and physiological function A 0.76-kb sequence of human noggin gene was cloned by polymerase chain reaction with the digestion site of Hind Ill and Xba I on the 5＇ end. The cloned fragment was reversely inserted into pCS2＋[Tal]-GFP plasmid, an neural cell-specific antisense eukaryotic expression vector. The plasmid expresses antisense for human noggin specifically in neurons, which may facilitate understanding of the physiological function of noggin.