A Novel Aptamer Lateral Flow Strip for the Rapid Detection of Gram-positive and Gram-negative Bacteria

作     者：Feifei Sun Chunlei Yan Qi Jia Wei Wu Yindi Cao 

作者机构：College of Food Science and EngineeringQingdao Agricultural UniversityQingdao 266109China Qingdao Institute of Special FoodQingdao Agricultural UniversityQingdao 266109China Laboratory of Quality and Safety Risk Assessment for Fruit(Qingdao)Ministry of Agriculture and Rural Aff airsQingdao 266109China National Technology Centre for Whole Process Quality Control of FSEN Horticultural Products(Qingdao)Qingdao 266109China Qingdao Science and Technology Service CentreQingdao 266001China 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2023年第7卷第1期

页      面：79-88页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0903[农学-农业资源与环境] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by the Breeding Plan of Shandong Provincial Qingchuang Research Team(2019-135) Qingdao Science and Technology Fund(21-1-4-sf-6-nsh) Postgraduate Innovation Program of Qingdao Agricultural University(QNYCX20028) High-level Talent Start-up fund from Qingdao Agricultural University(663/1121045) 

主　　题：Foodborne pathogens AuNPs-AMP antigens Vancomycin Aptamers Lateral flow strips 

摘      要：Diseases caused by foodborne pathogens are one of the main burdens of public health and seriously hinder global social and economic development.In this research,a novel lateral flow strip was successfully constructed for the simultaneous detection of Gram-positive and Gram-negative bacteria based on the fluidity and color aggregation effect of Au nanoparticles(AuNPs).The lateral flow strip first combined ampicillin(AMP)antigens with AuNPs to form AuNPs-AMP antigens as the first recognition molecule.Then vancomycin specifically recognizing Gram-positive bacteria,and aptamers specifi-cally recognizing Gram-negative bacteria were used as the second recognition molecules.Finally,Gram-positive bacteria and Gram-negative bacteria were detected rapidly using this sandwich mode.The strip can rapidly test the samples within 5 min.Using a lateral flow strip detector,the limit of detection was 4 CFU/mL,and the recovery rates in honey samples were 78.2-88.6%.The lateral flow strip constructed can detect foodborne pathogens quickly,accurately and e fficiently,which is of great significance for food safety detection.