Single and combined eff ects of fertilization,ectomycorrhizal inoculation,and drought on container-grown Japanese larch seedlings

作     者：Evgenios Agathokleous Mitsutoshi Kitao Masabumi Komatsu Yutaka Tamai Hisanori Harayama Takayoshi Koike Evgenios Agathokleous;Mitsutoshi Kitao;Masabumi Komatsu;Yutaka Tamai;Hisanori Harayama;Takayoshi Koike

作者机构：Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters(CIC-FEMD)Nanjing University of Information Science and TechnologyNanjing 210044JiangsuPeople’s Republic of China Research Center for Global Changes and Ecosystem Carbon Sequestration and MitigationSchool of Applied MeteorologyNanjing University of Information Science and TechnologyNanjing 210044JiangsuPeople’s Republic of China Hokkaido Research CenterForestry and Forest Products Research Institute(FFPRI)Forest Research and Management Organization7 HitsujigaokaSapporoHokkaido 062-8516Japan Department of Mushroom Science and Forest MicrobiologyForestry and Forest Products Research Institute(FFPRI)Matsunosato 1Tsukuba 305-8687Japan Research Faculty of AgricultureHokkaido UniversityKita 9 Nishi 9SapporoHokkaido 060-8589Japan Department of Plant EcologyForestry and Forest Products Research Institute(FFPRI)Matsunosato 1Tsukuba 305-8687Japan 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2023年第34卷第4期

页      面：1077-1094页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 

基　　金：supported by JSPS KAKENHI Grant Number JP17F17102 (to EA and MK) Startup Foundation for Introducing Talent of Nanjing University of Information Science and Technology (NUIST)(No. 003080) the Jiangsu Distinguished Professor program of the People’s Government of Jiangsu Province 

主　　题：Drought Ectomycorrhizae Fertilizer eff ect Oxidative stress Stressor interactions 

摘      要：Climate change can intensify drought in many regions of the world and lead to more frequent drought events or altered cycles of soil water status.Therefore,it is important to enhance the tolerance to drought and thus health,vigor,and success of transplantation seedlings used in the forestry by modifying fertilization and promoting mycorrhization.Here,we sowed seeds of Japanese larch(Larix kaempferi)in 0.2-L containers with 0.5 g(low fertilization;LF)or 2 g(high fertilization;HF)of slow-release fertilizer early in the growing season.One month later,we irrigated seedlings with non-sterilized ectomycorrhizal inoculum(ECM)or sterilized solution(non-ECM),and after about 2 months,plants were either kept well watered(WW;500 mL water/plant/week)or subjected to drought(DR;50 mL water per plant/week)until the end of the growing season.HF largely stimulated plant growth and above-and belowground biomass production,eff ects that are of practical signifi cance,but caused a small decrease in stomatal conductance(Gs 390)and transpiration rate(E 390),which in practice is insignifi cant.ECM treatment resulted in moderate inhibition of seedling growth and biomass and largely canceled out the enhancement of biomass and foliar K content by HF.DR caused a large decrease in CO 2 assimilation,and enhanced stomatal closure and induced senescence.DR also largely depleted foliar Mg and enriched foliar K.Although DR caused a large decrease in foliar P content in LF,it moderately increased P in HF.Likewise,DR increased foliar K in HF but not in LF,and decreased foliar P in ECM plants but not in non-ECM plants.Conversely,ECM plants exhibited a large enrichment in foliar P under WW and had a lower water potential under DR when grown in LF.These results indicate that the drought tolerance and health and vigor of Japanese larch seedlings can be modifi ed by soil fertility and soil microorganisms.This study provides a basis for new multifactorial research programs aimed at producing seedlings of superior quality for forestation under climate change.