Validation of a surgical model for posthepatectomy liver failure in rats

作     者：Andrea Lund Michelle Meier Kasper Jarlhelt Andersen Marie Ingemann Pedersen Anders Riegels Knudsen Jakob Kirkegård Frank Viborg Mortensen Andrea Lund;Michelle Meier;Kasper Jarlhelt Andersen;Marie Ingemann Pedersen;Anders Riegels Knudsen;Jakob Kirkeg?rd;Frank Viborg Mortensen

作者机构：Aarhus University HospitalDepartment of Upper Gastrointestinal and Hepato-Pancreato-Biliary SurgeryAarhus NDenmark 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2023年第6卷第3期

页      面：266-273页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：Arvid Nilssons Foundation Fabrikant Einar Willumsen Foundation Familien Hede Nielsen Foundation Kobmand Sven Hansen og Hustru Ina Hansen Foundation 

主　　题：90%liver resection general distress score liver failure post-hepatectomy liver failure rats 

摘      要：Background:The upper limit for liver resections in rats is approximately 90%.In the early postoperative phase,mortality increases.The aim of the present study was to validate the rat model of 90%partial hepatectomy(PH)as a model of post-hepatectomy liver failure(PHLF).Further,we wanted to test a quantitative scoring system as a detector of lethal outcomes caused by PHLF in rats.Methods:Sixty-eight rats were randomized to 90%PH,sham operation,or no sur-gery.Further,block randomization was performed based on time of euthanization:12,24,or 48 h after surgery.A general distress score(GDS)≥10 during the day or≥6 at midnight prompted early euthanization and classification as nonsurvivor.Animals euthanized as planned were classified as survivors.During euthanization,blood and liver tissue were collected,and liver-specific biochemistry was evaluated.Results:Based on the biochemical results,all animals subjected to 90%PH expe-rienced PHLF.Seventeen rats were euthanized due to irreversible PHLF.The GDS increased for nonsurvivors within 12–18 h after surgery.The mean time for euthaniza-tion was 27 h after surgery.Conclusion:Based on the GDS and liver-specific biochemistry,we concluded that the model of 90%PH seems to be a proper model for investigating PHLF in rats.As a high GDS is associated with increased mortality,the GDS appears to be valuable in detect-ing lethal outcomes caused by PHLF in rats.