Testing phaeophytinization as an index of ozone stress in trees

作     者：Evgenios Agathokleous Mitsutoshi Kitao Takayoshi Koike Evgenios Agathokleous;Mitsutoshi Kitao;Takayoshi Koike

作者机构：School of Applied MeteorologyNanjing University of Information Science and Technology(NUIST)Nanjing 210044People’s Republic of China Research Faculty of AgricultureHokkaido UniversitySapporoHokkaido 060-8589Japan Hokkaido Research CenterForestry and Forest Products Research Institute(FFPRI)Sapporo 062-8516Japan 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2023年第34卷第4期

页      面：1167-1174页

核心收录：

学科分类：0907[农学-林学] 07[理学] 0713[理学-生态学] 

基　　金：partly supported by the National Natural Science Foundation of China (No. 4210070867) the Research grant#201605 of the Forestry and Forest Products Research Institute (FFPRI),Japan KAKENHI grant#JP17F17102 of the Japan Society for the Promotion of Science (JSPS) 

主　　题：Biological stress Chemical eff ect Chlorophyll oxidation Environmental stimuli Phaeophytin 

摘      要：Ground-level ozone pollution is a menace for vegetation in the northern hemisphere,limiting photosynthetic pigments and suppressing photosynthesis in trees and other types of plants.Phaeophytinization is the process of converting chlorophylls into phaeophytins,for example by acidifi cation.Ozone is a highly oxidizing molecule and well known to degrade chlorophylls;however,the eff ect of ozone on phaeophytinization in leaves of higher plants is largely unknown.To reveal ozone eff ect on phaeophytinization and evaluate the potential of phaeophytinization as an index of ozone stress in trees,the absorbance at the optical density of 665 nm was measured before(OD_(665))and after(OD_(665a))acidifi cation in three independent experiments with nearly 30 conditions of ozone exposure.Both current ambient and elevated ozone widely aff ected phaeophytinization,as indicated by decreases or increases in the phaeophytinization quotient OD_(665)/OD_(665a).These eff ects were commonly moderate to large in magnitude and practically signifi cant,and occurred even in ozone-asymptomatic leaves.It emerges that the ozone eff ect on phaeophytinization is bimodal,likely depending on the intensity of ozone stress.These results indicate a promising feature of OD_(665)/OD_(665a)as a thorough index of ozone stress in the future,but further studies are needed to reveal the underlying biochemical mechanisms of the bimodal eff ect on phaeophytinization.