In Vitro Synthetic Seed Production of Potato under Different Fungicide Levels and Storage Intervals

作     者：Tahira Kalsoom Touqeer Ahmed Muhammad Azam Khan Mirza Hasanuzzaman Mukhtar Ahmed Stefaan P.O.Werbrouck 

作者机构：Department of HorticulturePMAS-Arid Agriculture UniversityRawalpindi46300Pakistan Department of AgronomySher-e-Bangla Agricultural UniversityDhaka1207Bangladesh Department of AgronomyPMAS-Arid Agriculture UniversityRawalpindi46300Pakistan Department of Plants and CropsGhent UniversityGhent9000Belgium 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2023年第92卷第8期

页      面：2429-2450页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Department of Horticulture Tissue Culture Laboratory PMAS-Arid Agriculture University 

主　　题：Synthetic seeds potato storage charcoal sodium alginate fungicide 

摘      要：Potato propagation through tubers and seed is an inefficient way of propagation thus we propose an alternative method in this study which is in vitro *** were raised from True Potato Seeds(TPS)in test tubes containing 10 mL of Murashige Skoog(MS)*** was done after every 21 days and after four subsequent subculturing,when there was an adequate quantity of stock material,uninodal cuttings of about 3-4 mm in length were prepared from in vitro proliferated potato plants by excising leaves of 1-2 mm on each side of the *** uninodal cuttings were cultured on 1/2 strength MS medium for root primordia *** selected uninodal cuttings with root primordia were encapsulated with sodium alginate solution(3.5%(w/v)),mixed with 1.25%charcoal and different concentration of fungicide(thiophanate methyl,sigma)to enhance the viability percentage of synthetic ***,these encapsulated uninodal cuttings were subjected to different storage intervals to check maximum *** showed that optimum dose for fungicide is 150 mg/L as it resulted in good viability percentage,shoot number,shoot length,rooting percentage,root number,root length and ideal for bead formation with sodium ***,results revealed that encapsulated uninodal cuttings can be stored at a temperature of 4℃ up to 45 days with a survival rate of 63.33%.Encapsulated uninodal cutting stored for 60 days showed a poor viability percentage of about 43.03%.It can be concluded that optimum dose of 150 mg/L with storage time of 45 days should be used to get a better outcome for synthetic potato seed production.