Molecular and functional analysis of chitin synthase genes in Chilo suppressalis(Lepidoptera:Crambidae)
作者机构：State&Local Joint Engineering Research Center of Green Pesticide Invention and Application/College of Plant ProtectionNanjing Agricultural UniversityNanjingChina College of Life and Environmental SciencesBiosciencesUniversity of ExeterPenrynUK
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2023年第30卷第3期
页 面：661-676页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：the National Natural Science Foundation of China(No.31972298&32172449)
主 题：Chilo suppressalis chitin synthase CRISPR/Cas9 pest control RNA interference
摘 要：The rice stem borer,Chilo suppressalis,has developed a high level of resistance to many of the compounds currently used for *** is therefore an urgent need to develop novel control methods for *** chitin synthases(CHS)have attracted interest as a potential target for insect pest ***,to date,CHS have not been characterized in *** CHS genes(CsCHSI and CsCHS2)were identified and cloned from *** transcript variants were identified for CsCHS1,CsCHSla and *** expression profiling showed that both transcripts of CsCHS1 are most highly expressed on the last day of each larval instar stage and show the highest expression levels in the *** contrast,CsCHS2 is predominantly expressed during the larval feeding stages and shows the highest expression levels in the *** of CsCHSI by RNA interference significantly inhibited the molting and pupation of ***,and knockdown of CsCHS2 significantly affected growth during the larval stage,but had no significant effect on the ***,knockout of CsCHS1 by CRISPR/Cas9 genome editing severely lowered the hatching rate,larval survivorship,pupation rate,and eclosion rate,but only larval survivorship at the GO generation was lowered after the knockout of *** results demonstrate that CsCHS1 and CsCHS2 play vital roles in the growth and development of ***,and so have potential as insecticidal targets for the control of this highly damaging pest.
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