High-throughput sequencing to evaluate the effects of methamphetamine on the succession of the bacterial community to estimate the postmortem interval

作     者：Shujuan Wang Wei Chen Yanjie Shang Lipin Ren Xiangyan Zhang Yadong Guo Changquan Zhang Shujuan Wang;Wei Chen;Yanjie Shang;Lipin Ren;Xiangyan Zhang;Yadong Guo;Changquan Zhang

作者机构：Department of Forensic ScienceSchool of Basic Medical SciencesCentral South UniversityChangshaChina 

出 版 物：《Forensic Sciences Research》 (法庭科学研究（英文）)

年 卷 期：2022年第7卷第4期

页      面：736-747页

核心收录：

学科分类：0301[法学-法学] 08[工学] 0838[工学-公安技术] 

基　　金：This study was supported by the National Natural Science Foundation of China[Grant No.81772026 820721140]. 

主　　题：Forensic sciences methamphetamine microbes bacterial community succession postmortem interval high-throughput sequencing 

摘      要：In forensic medical examinations,estimating the postmortem interval(PMI)is an important factor.Methamphetamine(MA)is a synthetic stimulant that is commonly abused,and estimation of the PMI after MA abuse has become one of the main tasks in forensic investigation.Microorganisms play a vital role in carrion decomposition.Analysing the bacterial succession patterns can be used as a forensic tool to estimate the PMI.The present study aimed to analyse bacterial succession changes during the decomposition of MA to estimate the PMI.We analysed bacterial communities in rabbits treated with three different concentrations of MA(0,22.5,and 90 mg/kg)under the natural conditions of 20°C and 70%humidity by sequencing 16S rRNA gene amplicons using the Illumina MiSeq system.We obtained 2374209 high-quality sequences and 2937 operational taxonomic units(OTUs).The relative abundances of the bacterial communities varied markedly in response to different MA concentrations.Interestingly,in response to the different concentrations of MA,Bacteroidetes became disparate in the rectum in the late PMI.Increased numbers of bacterial taxa were identified in the rectum and buccal cavity samples,except at the highest concentration of MA in the rectum samples when PMI was 0-h,than were present in live rabbits.Meanwhile,the PMI correlated significantly with bacterial succession at different taxonomic levels.Our results suggested that bacterial community succession could be used as a“microbial clockto estimate the PMI in cases of MA-related death;however,further study is required to gain a deeper understanding of this concept.