Scalp acupuncture Yikang therapy on Baihui(GV20),Sishencong(EX-HN1),Zhisanzhen,Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway

作     者：XUE Jianyi XU Jinyan HUANG Mao YU Wentao YAN Yihui YAN Yuanjie YIN Zhenjin LU Qian PENG Wanying YAN Siyang XUE Jianyi;XU Jinyan;HUANG Mao;YU Wentao;YAN Yihui;YAN Yuanjie;YIN Zhenjin;LU Qian;PENG Wanying;YAN Siyang

作者机构：Department of Acupuncture and MessageHebei University of Chinese MedicineShijiazhuang 050091China Department of Child Rehabilitationthe First Affiliated Hospital of Hebei University of Chinese MedicineShijiazhuang 050013China the second Outpatient DepartmentHebei University of Chinese MedicineShijiazhuang 050090China School of Basic MedicineHebei University of Chinese MedicineShijiazhuang 050200China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2023年第43卷第2期

页      面：337-342页

核心收录：

学科分类：1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by Key Projects of Scientific Research in Higher Education Institutions in Hebei Province:Study on the Mechanism of Scalp Acupuncture Yikang Therapy in Cerebral Palsy Rats Based on Notch Signal Pathway(ZD2020144) Doctoral Research Project of Hebei University of Chinese Medicine:Study on the Mechanism of Scalp Acupuncture Yikang Therapy in Cerebral Palsy Rats Based on Notch Signal Pathway(BSZ2020002)。 

主　　题：cerebral palsy scalp acupuncture Yikang therapy hippocampal tissue Notch signaling pathway 

摘      要：OBJECTIVE:To investigate the efficacy of scalp acupuncture Yikang therapy on Baihui(GV20),Sishencong(EX-HN1),Zhisanzhen,Niesanzhen,on neurobehavior in young rats with cerebral palsy based on Notch signaling pathway.METHODS:Thirty 7-day-old rats were randomly divided into sham,model and acupuncture,10 rats in each group.The cerebral palsy model was established by the accepted modeling method,the acupuncture group selectedBaihui(GV20),Sishencong(EX-HN1),ZhisanzhenandNiesanzhenfor intervention 24 h after the model was made.The body masses were recorded before and after the treatment,respectively.After the intervention,the rats were subjected to suspension experiment,slope experiment,tactile stimulation experiment and Morris water maze experiment.After the end of the experiment,the morphological changes of hippocampal histology were observed by hematoxylineosin(HE)staining under light microscope,and the expression of Notch1,Notch3 and Hes5 were detected by Western blot and quantitative real-time polymerase chain reaction(PCR).RESULTS:The changes in body mass of the rats in each group were different;in behavioral experiments,compared with the sham,the suspension time of the model was shortened,the slope experiment,tactile stimulation experiment,and escape latency time were prolonged,and the number of platform crossing was reduced in the model,compared with the model,the suspension time of the acupuncture was prolonged,the slope experiment,tactile stimulation experiment,and escape latency time were shortened,and the number of platform crossing times was increased;HE staining showed severe hippocampal damage in the model and reduced hippocampal damage in the acupuncture.Western Blot and real-time fluorescence quantitative PCR showed that the expression of Notch1,Notch3 and Hes5 were increased in the model and the expression of Notch1,Notch3,Hes5 in acupuncture were decreased.CONCLUSIONS:Scalp acupuncture Yikang therapy may improve neurobehavior and reduce brain injury in rats with cerebral palsy by downregulating the expression of Notch1,Notch3,and Hes5.