Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines

作     者：SUN Mengzhu ZHANG Yujie SONG Yafang GUO Jing WANG Yuhang XIN Chen GU Dongmei SUN Jianhua PEI Lixia SUN Mengzhu;ZHANG Yujie;SONG Yafang;GUO Jing;WANG Yuhang;XIN Chen;GU Dongmei;SUN Jianhua;PEI Lixia

作者机构：Department of acupunctureAffiliated Hospital of Nanjing University of Chinese MeidicneNanjing 210023China Department of acupunctureJiangsu Province Hospital of Chinese MedicineNanjing 210029China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2023年第43卷第3期

页      面：494-500页

核心收录：

学科分类：1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by the Research and Practice Innovation Program for Postgraduates of Jiangsu Province(Mechanism of Electroacupuncture Regulating Bile Acid Receptor to Alleviate Visceral Hypersensitivity in Irritable Bowel Syndrome,No.KYCX20_1469) the National Natural Science Fund(Mechanism of Electroacupuncture Regulating CRF-NLRP6 Inflammasome-Related Pathways in Intestinal Flora Immune Dialogue,No.81804193) the Leading Talents Project of Jiangsu Provincial Administration of TCM(Clinical Effect and Mechanism of Acupuncture and Moxibustion on Irritable Bowel Syndrome,No.SLJ0206) the Peak Talent Project of Jiangsu Province Hospital of TCM(Clinical Effect and Mechanism of Acupuncture and Moxibustion in the Treatment of Functional Gastrointestinal Diseases,No.y2018rc05)。 

主　　题：electroacupuncture irritable bowel syndrome intestinal barrier function tight junction proteins inflammatory cytokines 

摘      要：OBJECTIVE:To evaluate the effects and related mechanisms of electroacupuncture(EA)on irritable bowel syndrome(IBS).METHODS:Male C57BL/6 mice were randomly allocated into normal,model,and EA groups.Experimental IBS mice models were established by exposure to water avoidance stress(WAS).Mice in the EA group were treated with EA at bilateral Tianshu(ST 25)and Zusanli(ST 36)for 7 consecutive days,15 min each day.Abdominal withdrawal reflex(AWR)tests and intestinal motility tests were performed to evaluate visceral sensitivity and intestinal motility of mice.Expression levels of tight junction proteins(TJPs)and inflammatory cytokines in colon tissues were determined through immunofluorescence,real-time polymerase chain reactions(PCR)and Western blot assays.RESULTS:EA alleviated visceral hypersensitivity and intestinal hypermotility in WAS-induced IBS mice.Moreover,EA promoted the expression of zonula occludens(ZO)-1,claudin-1,and occludin while suppressing the expression of interleukin(IL)-8,interferon(IFN)-γ,and tumor necrosis factor(TNF)-αin water avoidance stress(WAS)-induced irritable bowel syndrome(IBS)mice.CONCLUSION:EA alleviated WAS-induced IBS in mice by promoting intestinal barrier functions and suppressing the expression of inflammatory cytokines.