Lysohexaenetides A and B,linear lipopeptides from Lysobacter *** 3655 identified by heterologous expression in Streptomyces

作     者：XU Qiushuang ZOU Haochen PAN Chen WANG Haoxin SHEN Yuemao LI Yaoyao XU Qiushuang;ZOU Haochen;PAN Chen;WANG Haoxin;SHEN Yuemao;LI Yaoyao

作者机构：Key Laboratory of Chemical Biology of Ministry of EducationSchool of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJinan 250012China State Key Laboratory of Microbial TechnologyShandong UniversityQingdao 266237China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2023年第21卷第6期

页      面：454-458页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.81573311 and 82173702) 

主　　题：Natural products Lysobacter Lipopeptides Heterologous expression 

摘      要：Lysobacter harbors a plethora of cryptic biosynthetic gene clusters(BGCs),albeit only a limited number have been analyzed to *** this study,we described the activation of a cryptic polyketide synthase(PKS)/nonribosomal peptide synthetase(NRPS)gene cluster(lsh)in Lysobacter *** 3655 through promoter engineering and heterologous expression in Streptomyces *** a result of this methodology,we were able to isolate two novel linear lipopeptides,lysohexaenetides A(1)and B(2),from the recombinant strain ***,we proposed the biosynthetic pathway for lysohexaenetides and identified LshA as another example of entirely iterative bacterial *** study highlights the potential of heterologous expression systems in uncovering cryptic biosynthetic pathways in Lysobacter genomes,particularly in the absence of genetic manipulation tools.