Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis

作     者：Hong-Cui Cao, Xiao-Ming Chen, Wei Xu, Department of Infectious Diseases, First Affiliated Hospital, Medical School of Zhejiang University Key Laboratory of Infectious Diseases of Ministry of Public Health, Hangzhou 310003, Zhejiang Province, China 

作者机构：Department of Infectious Disease First Affiliated Hospital Medical School of Zhejiang University Zhejiang Province China. 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2005年第11卷第46期

页      面：7364-7367页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Supported by a Health Foundation of Zhejiang Province  No.2004C083  2004B068 

主　　题：Platelet-activating factor Malondialdehyde Endotoxin rHPLC Viral hepatitis 

摘      要：AIM： To detect the plateletoactivating factor （PAF） and the plasma or serum levels of tumor necrosis factoroa （TNF-α） malondialdehyde （MDA）, endotoxin （ET） and to discuss their significance in various types of viral hepatitis. METHODS： PAF, TNF-α, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography （rHPLC）, bio-assay, ELISA, thiobarbituric acid （TBA）, and limulus lysate test （LLT）, respectively. RESULTS： The rHPLC was more sensitive and specific than bio-assay （r = 0.912, P〈0.01）. The plasma levels of PAF, TNF-α, MDA, and ET in patients with viral hepatitis were higher than those in controls （P〈0.01）. CONCLUSION： rHPLC is more reliable and accurate for the detection of PAF.