Chronic heat stress induces renal fibrosis and mitochondrial dysfunction in laying hens
作者机构：Division of Meat Animal and Poultry ResearchInstitute of Livestock and Grassland ScienceNational Agriculture and Food Research Organization(NILGS)2 IkenodaiTsukubaIbaraki 305‑0901Japan
出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)
年 卷 期：2023年第14卷第5期
页 面：2002-2011页
核心收录：
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
基 金：supported by Japan Society for the Promotion of Science KAKENHI Grant Number JP21K14966 to F.H the Environment Research and Technology Development Fund(JPMEERF20S11820)of the Environmental Restoration and Conservation Agency of Japan
主 题：cGAS-STING Heat stress Laying hens Mitochondrial DNA Renal fibrosis
摘 要：Background Heat stress in laying hens negatively affects egg production and shell quality by disrupting the homeo-stasis of plasma calcium and phosphorus *** the kidney plays an important role in calcium and phos-phorus homeostasis,evidence regarding the effect of heat stress on renal injury in laying hens is yet to be ***,the aim of this study was to evaluate the effects of chronic heat stress on renal damage in hens during laying *** A total of 16 white-leghorn laying hens(32 weeks old)were randomly assigned to two groups(n=8).One group was exposed to chronic heat stress(33°C for 4 weeks),whereas the other group was maintained at 24°*** Chronic heat exposure significantly increased plasma creatinine and decreased plasma albumin levels(P0.05).Heat exposure also increased renal fibrosis and the transcription levels of fibrosis-related genes(COLA1A1,αSMA,and TGF-β)in the *** results suggest that renal failure and fibrosis were induced by chronic heat exposure in laying *** addition,chronic heat exposure decreased ATP levels and mitochondrial DNA copy number(mtDNA-CN)in renal tissue,suggesting that renal mitochondrial dysfunction occurs under conditions of heat *** mitochondria leak mtDNAs into the cytosol and mtDNA leakage may activate the cyclic GMP-AMP synthase(cGAS)stimulator of interferon genes(STING)signaling *** results showed that chronic heat exposure activated the cGAS-STING pathway as indicated by increased expression of MDA5,STING,IRF7,MAVS,and NF-κB ***,the expression of pro-inflammatory cytokines(IL-12)and chemokines(CCL4 and CCL20)was upregulated in heat-stressed *** These results suggest that chronic heat exposure induces renal fibrosis and mitochondrial damage in laying *** damage by heat stress may activate the mtDNA-cGAS-STING signaling and cause subse-quent inflammation,which contributes to the progression of renal fibrosis and
维普期刊数据库