In silico Prediction and Analysis of Potential Off-Targets and Off-Target Mutation Detection in StERF3-Gene Edited Potato Plants

作     者：Hafiza Arooj Razzaq Siddra Ijaz Imran Ul Haq Faisal Saeed Awan 

作者机构：Centre of Agricultural Biochemistry and Biotechnology(CABB)University of AgricultureFaisalabad38040Pakistan Department of Plant PathologyUniversity of AgricultureFaisalabad38040Pakistan 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2023年第92卷第8期

页      面：2451-2460页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：CRISPR/Cas9 system potato CCTop algorithm scoring off-target edits 

摘      要：The imperative aspect of the CRISPR/Cas9 system is a short stretch of 20 nucleotides of gRNA that control the overall specifi*** to the small size,the chance of its multiple occurrences in the genome increases;however,a few mismatches are tolerated by the Cas9 endonuclease *** accurate and careful in silico-based off-target prediction while target selection is preferred to address the *** predictions are based on a comprehensive set of selectable ***,we investigated the possible off-target prediction and their screening in StERF3 gene-edited potato plants while developing StERF3-loss-of-function mutants using CRISPR/Cas9 *** 201 off-targets for the selected targets of the StERF3 gene were predicted,and 79 werefiltered as potential *** these 79,twenty-five off-targets showed scores with defined cut-off values0.5 and were analyzed in Sterf3-edited potato plants compared to wild-type *** off-targeting was found to have occurred in edited plants.