Single-cell transcriptomic analysis identifies a highly replicating Cd168+ þskeletal stem/progenitor cell population in mouse long bones

作     者：Rui-Cong Hao Zhi-Ling Li Fei-Yan Wang Jie Tang Pei-Lin Li Bo-Feng Yin Xiao-Tong Li Meng-Yue Han Ning Mao Bing Liu Li Ding Heng Zhu Rui-Cong Hao;Zhi-Ling Li;Fei-Yan Wang;Jie Tang;Pei-Lin Li;Bo-Feng Yin;Xiao-Tong Li;Meng-Yue Han;Ning Mao;Bing Liu;Li Ding;Heng Zhu

作者机构：Basic Medical College of Anhui Medical UniversityHefeiAnhui 230032China Beijing Institute of Radiation MedicineBeijing 100850China Beijing Institute of Basic Medical SciencesBeijing 100850China State Key Laboratory of Experimental HematologyDepartment of HematologyFifth Medical Center of Chinese PLA General HospitalBeijing 100071China Air Force Medical CenterPLABeijing 100142China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报(英文版))

年 卷 期：2023年第50卷第9期

页      面：702-712页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2022YFA1104100,2022YFA1103500) the National Natural Sciences Grants China(82172388,82372373,81871771) the Beijing Natural Sciences Foundation(7222123,L212065) 

主　　题：Skeletal stem cell scRNA-seq Bone development Fracture healing Bone regeneration Development 

摘      要：Skeletal stem/progenitor cells(SSPCs)are tissue-specific stem/progenitor cells localized within skeletons and contribute to bone development,homeostasis,and ***,the heterogeneity of SSPC populations in mouse long bones and their respective regenerative capacity remain to be further *** this study,we perform integrated analysis using single-cell RNA sequencing(scRNA-seq)datasets of mouse hindlimb buds,postnatal long bones,and fractured long *** analyses reveal the heterogeneity of osteochondrogenic lineage cells and recapitulate the developmental trajectories during mouse long bone *** addition,we identify a novel Cd168þSSPC population with highly replicating capacity and osteochondrogenic potential in embryonic and postnatal long ***,the Cd168þSSPCs can contribute to newly formed skeletal tissues during fracture ***,the results of multicolor immunofluorescence show that Cd168þSSPCs reside in the superficial zone of articular cartilage as well as in growth plates of postnatal mouse long *** summary,we identify a novel Cd168þSSPC population with regenerative potential in mouse long bones,which adds to the knowledge of the tissuespecific stem cells in skeletons.