Feeding citrus flavonoid extracts decreases bacterial endotoxin and systemic inflammation and improves immunometabolic status by modulating hindgut microbiome and metabolome in lactating dairy cows

作     者：Yuchao Zhao Shiqiang Yu Liuxue Li Huiying Zhao Yuqin Li Linshu Jiang Ming Liu Yuchao Zhao;Shiqiang Yu;Liuxue Li;Huiying Zhao;Yuqin Li;Linshu Jiang;Ming Liu

作者机构：Beijing Key Laboratory of Dairy Cow NutritionAnimal Science and Technology CollegeBeijing University of AgricultureBeijing102206China Beijing Beinong Enterprise Management Co.Ltd.Beijing102206China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第13卷第2期

页      面：386-400页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：funded by the Categorized Development Project 2022(Start-up Foundation for Talent Introduction,Beijing University of Agriculture 5066516004/003) the China Postdoctoral Science Foundation(2022M710181) 

主　　题：Citrus flavonoid Dairy cow Fecal microbiota Systemic inflammation Volatile fatty acid 

摘      要：The objectives of this study were to determine the effects of dietary supplementation with citrus flavonoid extracts(CFE)on milk performance,serum biochemistry parameters,fecal volatile fatty acids,fecal microbial community,and fecal metabolites in dairy *** multiparous lactating Holstein cows were used in a replicated 4×4 Latin square design(21-day period).Cows were fed a basal diet without addition(CON)or basal diet with added CFE at 50(CFE50),100(CFE10),and 150 g/d(CFE150).Feeding CFE up to 150 g/d increased milk yield and milk lactose *** CFE linearly decreased milk somatic cell *** cytokines interleukin-1β(IL-1β),IL-2,IL-6,and tumor necrosis factor-α(TNF-α)concentrations decreased linearly as the levels of CFE *** in CFE150 had lower serum lipopolysaccharide and lipopolysaccharide binding protein compared with *** results indicate feeding CFE decreased systemic inflammation and endotoxin levels in dairy ***,feeding CFE linearly increased the concentrations of total volatile fatty acids,acetate,and butyrate in *** relative abundances of beneficial bacteria Bifidobacterium spp.,Clostridium coccoides-Eubacterium rectale group,and Faecalibacterium prausnitzii in feces increased linearly with increasing CFE *** diversity and community structure of fecal microbiota were un-affected by CFE ***,supplementing CFE reduced the relative abundances of genera Ruminococcus_torques_group,Roseburia,and Lachnospira,but increased genera Bacteroides and *** analysis showed that supplementary CFE resulted in a significant modification in the fecal metabolites *** with CON,fecal naringenin,hesperetin,hippuric acid,and sphingosine concentrations were greater in CFE150 cows,while fecal GlcCer(d18:1/20:0),Cer(d18:0/24:0),Cer(d18:0/22:0),sphinganine,and deoxycholic acid concentrations were less in CFE150 *** pathway analysis s