Copy number variation of B1 controls awn length in wheat
作者机构：College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China State Key Laboratory of Crop BiologyCollege of Life SciencesShandong Agricultural UniversityTai’an 271018ShandongChina Novogene Bioinformatics Technology Co.Ltd.Beijing 100015China
出 版 物：《The Crop Journal》 (作物学报（英文版）)
年 卷 期：2023年第11卷第3期
页 面：817-824页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学]
基 金：supported by the National Key Research and Development Program of China(2018YFD0300501)。
主 题：Wheat Awn Awnless,B1 gene Copy number variation
摘 要：Wheat awns contribute to photosynthesis and grain production.In this study,an F2population and F2:3families from a cross between the awned line 7D12 and the Chinese awnless variety Shiyou 20(SY20)were used to identify loci associated with awn length.Bulked-segregant RNA sequencing and linkage mapping identified a single dominant locus in a 0.3 cM interval on chromosome 5AL.Five genes were in the interval,including the recently cloned awn inhibitor B1.Although a single copy of the B1 gene was detected in 7D12,SY20 carried five copies of the gene.Increased copy number of B1 in SY20enhanced gene expression.Based on sequence variation among the promoter regions of five B1 gene copies in SY20,two dominant markers were developed and found to cosegregate with B1 in a population of 931 wheat accessions.All 77 awnless accessions harbored sequence variations in the B1 promoter regions similar to those of SY20 and thus carried multiple copies of the gene,whereas 15 randomly selected awned wheats carried only one copy.These results suggest that an increase in copy number of the B1 gene is associated with inhibition of awn length.
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