Chemical compositions,cytotoxicity and antioxidant activity of the endophytic fungus Fusarium napiforme isolated from Psidium guajava

作     者：Chutulo EC Chalannavar RK Pramod Kumar P 

作者机构：Department of Studies&Research in MicrobiologyMangalore UniversityJnana KaveriP.G.CentreChikka AluvaraKodagu-571232KarnatakaIndia Department of BiosciencesMangalore UniversityMangalagangotri-574199KarnatakaIndia Department of BiologyWolaita Sodo UniversityWolaita SodoEthiopia Department of Applied BotanyMangalore UniversityMangalagangotri-574199KarnatakaIndia Department of BiochemistryCSIR-Central Food Technological Research InstituteMysuru-570020KarnatakaIndia 

出 版 物：《Studies in Fungi》 (真菌研究（英文）)

年 卷 期：2020年第5卷第1期

页      面：332-352页

学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1203[管理学-农林经济管理] 0903[农学-农业资源与环境] 0706[理学-大气科学] 0901[农学-作物学] 0902[农学-园艺学] 0836[工学-生物工程] 0713[理学-生态学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：flavonoids GC-MS analysis lung adenocarcinoma cells mouse embryo fibroblast cells MTT assay phenolics 

摘      要：The bioactive secondary metabolites from the endophytic fungus Fusarium napiforme was evaluated for the cytotoxic effect and antioxidant *** total antioxidant capacity(TAC)of the extract was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH),phosphomolybdate,and reducing power assay *** cytotoxicity of the extract was evaluated against lung adenocarcinoma(A549)cells and mouse embryo fibroblast(NIH3T3)cells by methyl thiazolyl tetrazolium(MTT)*** major composition of the crude extract was identified by the Gas Chromatography-Mass Spectrometry(GC-MS)*** of the endophyte crude extract revealed a high amount of the total flavonoid content(TFC)and total phenolic content(TPC).The extract showed high cytotoxic activity against the A549 cell line with the mean cytotoxicity of 69.74±0.49%.The extract did not show any cytotoxic effect against the NIH3T3 cell *** extract exhibited high antioxidant activity as a function of the *** a test concentration of 1 mg ml-1,the extract showed the highest inhibition against DPPH radical at 75.4607%±0.47688,ferric ion reducing power at 0.882±0.0120,and 255.434±21.404 AAE/g of the extract by phosphomolybdenum assay(PMA).There is a significant correlation between TPC and antioxidant activity at p*** correlation between reducing power and DPPH is significant at p*** major types of bioactive compounds identified by the GC-MS have shown the presence of nine major *** result strongly exhibits that the endophyte *** can be a potential source for the formulation of natural anticancer drugs and protecting the body from oxidative damages.