Insecticidal Potential of α-Pinene and β-Caryophyllene against Myzus persicae and Their Impacts on Gene Expression
作者机构：Institute of Molecular Biology and Biotechnology(IMBB)The University of LahoreLahore54000Pakistan Institute of Pharmaceutical SciencesUniversity of Veterinary and Animal SciencesLahore54000Pakistan Deparment of Plant PathologyUniversity of OkaraOkara56300Pakistan Deparment of BioinformaticsUniversity of OkaraOkara56300Pakistan Department of Soil ScienceThe Islamia University of BahawalpurBahawalpur63100Pakistan Department of ZoologyThe Islamia University of BahawalpurBahawalpur(Bahawalnagar Campus)63100Pakistan The Steve and Cindy Rasmussen Institute for Genomic MedicineNationwide Children’s HospitalColumbusOhio43205USA Department of Botany and MicrobiologyCollege of ScienceKing Saud UniversityRiyadh11451Saudi Arabia
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2023年第92卷第7期
页 面：1943-1954页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：funded by the Researchers Supporting Project Number(RSP2023R123) King Saud University Riyadh Saudi Arabia
主 题：Fumigation M.persicae gene expression real time PCR α-pinene β-caryophyllene
摘 要：Myzus persicae(***)is now considered a threat to agricultural crops due to economic *** synthetic insecticides applied every year against ***,are reported to be unsafe for environment,humans,and beneficial ***,several species of Myzus have been found to develop resistance due to over application of these ***,it is required to find some novel insecticide that would be safe for the environment as well as for *** the current study,two major pure constituentsα-pinene andβ-caryophyllene were evaluated for their insecticidal potential against *** using a fumigant toxicity ***,impact ofα-pinene andβ-caryophyllene on expression of five different genes,e.g.,HSP 60,FPPS I,OSD,TOL and ANT responsible for reproduction,dispersion,and growth of *** has also been *** perform fumigant toxicity assay,five different concentrations(3.5,4,4.5,5 and 6μL L−1)ofα-pinene andβ-caryophyllene were *** concentration(LC)was calculated,and gene expression studies were executed through qRT PCR at LC30 ofα-pinene andβ-*** constituents demonstrated excellent fumigant toxicity effects against *** at all five ***,α-pinene shows significantly better results(98%)as compared toβ-caryophyllene(80%)after 72 h at 6μL L−1 of *** highest upregulation in expression was demonstrated at LC30 dose ofα-pinene in five in three out of five genes understudy(TOL,ANT,and FPPS I).Conversely,two genes HSP 60 and OSD demonstrated downregulation at LC30 dose ofβ-***,our results highlighted the promising insecticidal potential of both compoundsα-pinene andβ-caryophylleneby interfering with the reproduction and development related processes in ***,allowing us to recommend the phytoconstituents under investigation as an ecofriendly alternative to synthetic insecticides.
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