A pH-Regulated Colorimetric Sensor Array for Discrimination of Biothiols Based on Two Different-Sized β-Cyclodextrin-Functionalized AuNPs

作     者：Hang Li Chao Zhao Shengnan Wei Xingxing Liu Juan Li Juan Wang 

作者机构：School of Public HealthJilin UniversityChangchun 130021China The Department of Cadre WardThe First Hospital of Jilin UniversityChangchun 130021China 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2023年第7卷第2期

页      面：101-109页

核心收录：

学科分类：081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 080202[工学-机械电子工程] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 0802[工学-机械工程] 10[医学] 

基　　金：the financial support from the National Natural Science Foundation of China(82073603) Jilin Province Science and Technology Development Plan Item(20200602010ZP) Health Commission of Jilin Province(2020Q011) Norman Bethune Health Science Center of Jilin University(2020B39) 

主　　题：Colorimetric sensor array Biothiols β-Cyclodextrin-functionalized AuNPs Principal component analysis 

摘      要：The abnormal changes of biothiols are directly related to health condition of human body,and the effective identification and quantification of biothiols is of great significance for screening and diagnosis of *** study described the development of a p H-regulated colorimetric sensor array for discrimination of five kinds of biothiols including cysteine(Cys),glutathione(GSH),homocysteine(Hcy),cysteamine(CA)and N-acetylcysteine(NAC).The proposed sensor array was established using 5-nm-and 20-nm-sizedβ-cyclodextrin-functionalized gold nanoparticles(β-CD@Au NPs)as nonspecific receptor and signal transduction *** to the different binding affinity between biothiols andβ-CD@Au NPs in various p H environments,the different aggregation behaviors of nanoparticles produced unique colorimetric response patterns,which were able to be distinguished by bare eyes and UV-vis ***,principal component analysis(PCA)and hierarchical cluster analysis(HCA)were employed for pattern recognition and generated a clustering map for a clear differentiation of biothiols at the level ofμmol/***,it can be proved that the method was successfully applied to the analysis of biothiols in human urine samples.