Plasmonic polydopamine-modified TiO_(2)nanotube substrates for surface-assisted laser desorption/ionization mass spectrometry imaging

作     者：Dong Chen Mingyi Du Yudi Huang Yizhu Xu Yingying Chen Lianlian Ma Qingrong Xie Xinhai Zhu Zilong Chen Hanhong Xu Xinzhou Wu Zhibin Yin Dong Chen;Mingyi Du;Yudi Huang;Yizhu Xu;Yingying Chen;Lianlian Ma;Qingrong Xie;Xinhai Zhu;Zilong Chen;Hanhong Xu;Xinzhou Wu;Zhibin Yin

作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of EducationSouth China Agricultural UniversityGuangzhou 510642China Agro-biological Gene Research CenterGuangdong Academy of Agricultural SciencesGuangzhou 510640China Instrumental Analysis and Research CenterSun Yat-sen UniversityGuangzhou 510275China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2023年第16卷第2期

页      面：3028-3039页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 070205[理学-凝聚态物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学] 

基　　金：the National Natural Science Foundation of China(Nos.31901911 and 21904142) the Natural Science Foundation of Guangdong Province(Nos.2019A1515011521 and 2022A1515011385) supported by the Young Talent Support Project of Guangzhou Association for Science and Technology(No.QT20220101031). 

主　　题：TiO_(2)nanotube surface-assisted laser desorption/ionization(SALDI) mass spectrometry imaging latent fingerprint tissue imaging 

摘      要：Mass spectrometry imaging(MSI)has made the spatio-chemical characterization of a broad range of small-molecule metabolites within biological tissues possible.However,available matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)suffers from severe background interferences in low-mass ranges and inhomogeneous matrix deposition.Thus,surface-assisted LDI-MS(SALDI-MS)has been an attractive alternative for high-sensitivity detection and imaging of small biomolecules.In this study,we construct a new composite substrate,hydrophobic polydopamine(hPDA)-modified TiO_(2)nanotube(TDNT)coated with plasmonic gold nanoparticle(AuNP-hPDA-TDNT),as a dual-polarity SALDI substrate using an easy and cost-effective fabrication approach.Benefitting from the synergistic effects of TDNT semiconductor and plasmonic PDA modification,this SALDI substrate exhibits superior performance for dual-polarity detection of a vast diversity of small molecules.Highly reduced background interferences,lower detection limits,and spot-to-spot repeatability can be achieved using AuNP-hPDA-TDNT substrates.Due to its unique imprinting performance,various metabolites and lipids can be visualized within jatropha integerrima petals,ginkgo leaves,strawberry fruits,and latent fingerprints.More valuably,the universality of this matrix-free substrate is demonstrated for mapping spatial distribution of lipids within mouse brain tissue sections.Considered together,this AuNP-hPDA-TDNT material is expected to be a promising SALDI substrate in various fields,especially in nanomaterial development and life sciences.