Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis
作者机构：Department of MicrobiologySchool of Basic Medical SciencesCapital Medical UniversityBeijing100069China Department of NeurosurgeryCapital Medical University Sanbo Brain HospitalBeijing100093China Center of EpilepsyBeijing Institute for Brain DisordersBeijing100093China
出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)
年 卷 期：2023年第38卷第1期
页 面：66-74页
核心收录：
学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
基 金：funded by the National Key Research and Development Plan of China(2021YFC2300202) the National Natural Science Foundation of China(U1902210,81871641,81972979,82172266,81902048) the Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan(IDHT20190510) the Beijing Key Laboratory of Emerging Infectious Diseases(NO.DTKF202103).
主 题：Organoid 3D cell culture Testicular organoid model Zika virus(ZIKV)
摘 要：Zika virus(ZIKV)poses a serious threat to global public health due to its close relationship with neurological and male reproductive damage.However,deficiency of human testicular samples hinders the in-depth research on ZIKV-induced male reproductive system injury.Organoids are relatively simple in vitro models,which could mimic the pathological changes of corresponding organs.In this study,we constructed a 3D testicular organoid model using primary testicular cells from adult BALB/c mice.Similar to the testis,this organoid system has a blood-testis barrier(BTB)-like structure and could synthesize testosterone.ZIKV tropism of testicular cells and ZIKV-induced pathological changes in testicular organoid was also similar to that in mammalian testis.Therefore,our results provide a simple and reproducible in vitro testicular model for the investigations of ZIKV-induced testicular injury.
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