Metabolomic profiles in a green alga (Raphidocelis subcapitata) following erythromycin treatment: ABC transporters and energy metabolism

作     者：Jiezhang Mo Zhihua Ma Shiwei Yan Napo KM Cheung Fangshe Yang Xiunan Yao Jiahua Guo 

作者机构：Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying CapacityCollege of Urban and Environmental SciencesNorthwest UniversityXi’an 710127China State Key Laboratory of Marine Pollution and Department of ChemistryCity University of Hong KongKowloonHong KongChina 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第124卷第2期

页      面：591-601页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 0815[工学-水利工程] 0703[理学-化学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No. 42101077) The Key Research and Development Program of Shaan Xi Province (No. 2020SF-387) ShaanXi Thousand Talent Program for Young Outstanding Scientists (No. 334041900007) 

主　　题：Hormesis Metabolome ABC transporters DNA replication Microalgae Macrolide antibiotic 

摘      要：A recent study showed that erythromycin(ERY)exposure caused hormesis in a model alga(Raphidocelis subcapitata)where the growth was promoted at an environmentally realistic concentration(4μg/L)but inhibited at two higher concentrations(80 and 120μg/L),associated with opposite actions of certain signaling pathways(e.g.,xenobiotic metabolism,DNA replication).However,these transcriptional alterations remain to be investigated and verified at the metabolomic level.This study uncovered metabolomic profiles and detailed toxic mechanisms of ERY in R.subcapitata using untargetedmetabolomics.Themetabolomic analysis showed that metabolomic pathways including ABC transporters,fatty acid biosynthesis and purine metabolism were associated with growth promotion in algae treated with 4μg/L ERY.An overcompensation was possibly activated by the low level of ERY in algae where more resources were reallocated to efficiently restore the temporary impairments,ultimately leading to the outperformance of growth.By contrast,algal growth inhibition in the 80 and 120μg/L ERY treatments was likely attributed to the dysfunction of metabolomic pathways related to ABC transporters,energy metabolism and metabolism of nucleosides.Apart from binding of ERY to the 50S subunit of ribosomes to inhibit protein translation as in bacteria,the data presented here indicate that inhibition of protein translation and growth performance of algae by ERY may also result from the suppression of amino acid biosynthesis and aminoacyl-tRNA biosynthesis.This study provides novel insights into the dose-dependent toxicity of ERY on R.subcapitata.