Proteomics analysis provides novel biomarkers and therapeutic target candidates in the treatment of the Huang-Pu-Tong-Qiao formula in an AD rat mode
作者机构：School of Integrated Chinese and Western MedicineAnhui University of Chinese MedicineHefei230012China Anhui Province Key Laboratory of Chinese Medicinal FormulaAnhui University of Chinese MedicineHefei230012China
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2023年第47卷第6期
页 面：1265-1277页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 10[医学]
基 金：the National Natural Science Foundation of China(Grant No.81873351 and 82205235) the Academic Support Program for High School Discipline(Professional)Top Talent(Grant No.gxbjZD2022024) the Key Project Foundation of Natural Science Research in Universities of Anhui Province in China(grant Nos.KJ2020A0439 and KJ2020A0416) the Talent Support Program of Anhui University of Traditional Chinese Medicine(2022rcyb028).
主 题：Alzheimer’s disease ITRAQ Huang-Pu-Tong-Qiao
摘 要：Background:Huang-Pu-Tong-Qiao formula(HPTQ),a traditional Chinese herbal formula,has a variety of pharmacological effects.It has been used to treat Alzheimer’s disease(AD)for decades.This study aimed to screen differentially expressed proteins in the hippocampus of AD model rats treated with HPTQ.Proteomic studies of the effects of HPTQ on AD are key to understanding the therapeutic mechanisms of HPTQ and identifying potential therapeutic targets.Methods:We hence used the isobaric tags for relative and absolute quantification(ITRAQ)approach to investigate the differentially expressed proteins in the hippocampus of AD model rats before and after HPTQ administration and to identify the potential therapeutic target proteins of HPTQ.In this study,the learning and memory abilities of AD rats were examined by the Morris water maze test.After HPTQ administration,the differentially expressed proteins in the hippocampus of AD rats were quantified and analyzed in silico.Furthermore,western blotting was used to verify the expression of related proteins.Results:The Morris water maze results showed that HPTQ could improve the learning and memory ability of AD model rats.The proteomics analysis results showed that 57 proteins were differentially expressed,of which 35 were up-regulated and 22 were down-regulated.Bioinformatics analysis indicated that proteins with altered expression after HPTQ treatment were involved in several biological processes that have the potential to exert neuroprotective effects.These included promoting the translation of ribosomes,improving the deposition of amyloid-beta(Aβ),regulating autophagy,regulating neuronal synaptic function and plasticity,and alleviating oxidative stress.Conclusion:In conclusion,we identified several potential therapeutic target proteins and related mechanistic pathways of HPTQ in the treatment of AD,laying the foundation for further investigation of the therapeutic effects of HPTQ.
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